{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T07:56:54Z","timestamp":1777103814496,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T00:00:00Z","timestamp":1776816000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cryopreservation is an essential component of assisted reproductive technologies in dogs. However, post\u2010thaw semen quality is often compromised by cryo\u2010induced oxidative stress, membrane disruption, and DNA damage, which ultimately reduce fertility outcomes. Conventional egg yolk (EY)\u2010based extenders provide partial protection during freezing but pose biosecurity rick and exhibit batch\u2010to\u2010batch variability. Gum arabic (GA), a plant\u2010derived polysaccharide with cryoprotective and antioxidant properties, has emerged as a potential alternative. The present study evaluated the comparative efficacy of EY and GA extenders, with and without microencapsulation and \u03b1\u2010lipoic acid (ALA) supplementation, on post\u2010thaw semen quality in dogs. Semen was collected from six sexually mature Labrador retrievers, with three ejaculates obtained from each dog. Semen samples were cryopreserved in straws under three treatment conditions: extender alone (Treatment 1), extender with microencapsulation (Treatment 2), and extender with microencapsulation plus ALA (Treatment 3). Post\u2010thaw evaluation included sperm motility, viability, acrosomal integrity, hypo\u2010osmotic swelling test (HOST), DNA fragmentation index (DFI), mitochondrial membrane potential (MMP), and total antioxidant capacity (TAC). The results indicated that microencapsulation and ALA supplementation improved several post\u2010thaw sperm quality parameters. Treatment 3 showed the highest values of sperm viability, plasma membrane integrity, acrosomal integrity, MMP, and total antioxidant capacity in both extenders. Sperm motility and DFI did not differ significantly among treatments, although numerically lower DFI values were observed in GA\u2010based treatments. In conclusion, the combined use of GA extender, sperm microencapsulation, and ALA supplementation may enhance the preservation of canine sperm functional integrity during cryopreservation. Further studies evaluating fertility outcomes are required to confirm the practical applicability of these approaches in canine reproductive technologies.<\/jats:p>","DOI":"10.1111\/rda.70210","type":"journal-article","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T14:04:39Z","timestamp":1776866679000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of Egg Yolk and Gum Arabic Extenders With Microencapsulation and \u03b1\u2010Lipoic Acid Supplementation for Canine Semen Cryopreservation"],"prefix":"10.1111","volume":"61","author":[{"given":"Shubham","family":"Mandhale","sequence":"first","affiliation":[{"name":"Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University  Nagpur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0382-725X","authenticated-orcid":false,"given":"Mahesh","family":"Gupta","sequence":"additional","affiliation":[{"name":"Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University  Nagpur Maharashtra India"},{"name":"ICAR\u2010National Institute of Abiotic Stress Management  Pune India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jayant Pandurang","family":"Korde","sequence":"additional","affiliation":[{"name":"Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University  Nagpur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krushna Bibichand","family":"Bahiram","sequence":"additional","affiliation":[{"name":"Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University  Nagpur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachin Wasudeorao","family":"Bonde","sequence":"additional","affiliation":[{"name":"Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University  Nagpur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoj","family":"Patil","sequence":"additional","affiliation":[{"name":"Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University  Nagpur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohit","family":"Rathod","sequence":"additional","affiliation":[{"name":"Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University  Nagpur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pratik","family":"Kokate","sequence":"additional","affiliation":[{"name":"Nagpur Veterinary College Maharashtra Animal and Fishery Sciences University  Nagpur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nitin Eknath","family":"Gade","sequence":"additional","affiliation":[{"name":"College of Veterinary Science and Animal Husbandary, DSVCKV, Anjora, Durg  Chattisgarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756287216652779"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.2014.32.1.1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2017.09.008"},{"key":"e_1_2_11_5_1","unstructured":"Bambode K.2021.\u201cEvaluation of Seminal Antioxidative Potential and Sperm Morphological Characteristics in Cow and Buffalo Bull.\u201d M.V.Sc Thesis Maharashtra Animal and Fishery Sciences University (MAFSU) Nagpur India."},{"issue":"3","key":"e_1_2_11_6_1","first-page":"307","article-title":"Effect of \u03b1\u2010Lipoic Acid Supplementation to Extender on Quality of Frozen\u2010Thawed Bull Semen","volume":"21","author":"B\u00fcy\u00fckleblebici O.","year":"2015","journal-title":"Kafkas \u00dcniversitesi Veteriner Fak\u00fcltesi Dergisi"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13010111"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(93)90230-3"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.20546\/ijcmas.2020.908.276"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.09.011"},{"key":"e_1_2_11_11_1","doi-asserted-by":"crossref","unstructured":"Gangwar C. S. D.Kharche R.Pourauchottamane R.Ranjan andM.Chatli.2023.\u201cHigh End Workshop: Advances in Artificial Insemination in Goat.\u201d InProceedings of SERB Workshop Central Institute for Research on Goats (CIRG) Makhdoom 13 85\u201386.","DOI":"10.59317\/9789395763172"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-26960-y"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.445"},{"key":"e_1_2_11_14_1","unstructured":"Hoffmann B. andG.Schuler.2004.Semen Collection Preservation and Artificial Insemination in the Dog. In:Scientific Proceedings of the 29th World Small Animal Veterinary Congress (WSAVA) 10th European Congress (FECAVA) and 7th Hellenic Congress3:754\u2013757."},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10111780"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2019.106166"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.29005\/ijcp.2018.10.1.037\u2010040"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13028-020-00530-6"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2011.04.006"},{"issue":"8","key":"e_1_2_11_20_1","first-page":"1277","article-title":"Collection and Evaluation of Dog Semen: A Mini Review","volume":"12","author":"Meena A. 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