{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T12:07:50Z","timestamp":1779970070073,"version":"3.53.1"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The luteinizing hormone\/choriogonadotropin receptor (LHCGR) gene is potentially a useful genetic marker for cattle reproductive status. This study aimed to investigate the impact of the LHCGR gene's ss52050737 polymorphism on age at first calving (AFC), calving interval (CI), and ovarian status using the TaqMan PCR assay. AFC was categorized into 25\u201334 and 35\u201345\u2009months, while CI was grouped into 330\u2013410 and 411\u2013800\u2009days. Ovarian status was classified as active or inactive ovaries. Blood samples were collected from cows using EDTA\u2010containing vacutainer tubes and stored at \u221220\u00b0C until DNA extraction and analysis. Three genotypes (GG, GT, and TT) were identified for the LHCGR gene SNP (ss52050737). The homozygous GG genotype was more frequent in cows with a CI of 330\u2013410\u2009days, and the genotype distribution showed a statistically significant association (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.018) in cows with CI 330\u2013410 and 411\u2013800\u2009days. On the other hand, the GG genotype was not significantly different in relation to AFC and ovarian status (active or inactive). These findings suggest that the ss52050737 polymorphism of the LHCGR gene has an impact on calving interval, and the G allele is associated with the CI of 330\u2013410\u2009days.\n                  <\/jats:p>","DOI":"10.1111\/rda.70212","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T09:17:53Z","timestamp":1777540673000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Impact of Single Nucleotide Polymorphism Ss52050737 in Luteinizing Hormone\/Choriogonadotropin Receptor Gene on Some Reproductive Traits in Friesian Cows"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7114-4325","authenticated-orcid":false,"given":"Samar Samir","family":"Youssef","sequence":"first","affiliation":[{"name":"Microbial Biotechnology Department Biotechnology Research Institute, National Research Centre  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6536-7538","authenticated-orcid":false,"given":"Eman Abd El\u2010Razek","family":"Abbas","sequence":"additional","affiliation":[{"name":"Microbial Biotechnology Department Biotechnology Research Institute, National Research Centre  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9726-2936","authenticated-orcid":false,"given":"Ahmed S. A.","family":"Sosa","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction &amp; AI Veterinary Research Institute, National Research Centre  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3283-2309","authenticated-orcid":false,"given":"Mahmoud H.","family":"Hasanain","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction &amp; AI Veterinary Research Institute, National Research Centre  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2098-6463","authenticated-orcid":false,"given":"Sally","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction &amp; AI Veterinary Research Institute, National Research Centre  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5595-0482","authenticated-orcid":false,"given":"Mohamed M. M.","family":"El\u2010Sokary","sequence":"additional","affiliation":[{"name":"Department of Theriogenology Faculty of Veterinary Medicine, Benha University  Kaliobeya Egypt"},{"name":"Higher Colleges of Technology, Faculty of Health Science  Abu Dhabi UAE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4068-362X","authenticated-orcid":false,"given":"Mahmoud F.","family":"Nawito","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction &amp; AI Veterinary Research Institute, National Research Centre  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0482-1207","authenticated-orcid":false,"given":"Karima Gh. M.","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction &amp; AI Veterinary Research Institute, National Research Centre  Giza Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"143","article-title":"Polymorphisms of the Bovine Luteinizing Hormone\/Choriogonadotropin Receptor (LHCGR) Gene and Its Association With Ovarian Follicular Cysts","volume":"168","author":"Abdi Z.","year":"2017","journal-title":"Revue de M\u00e9decine V\u00e9t\u00e9rinaire"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.19080\/JDVS.2018.05.555655"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/10495398.2022.2044346"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0244825"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3934\/agrfood.2018.3.306"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.4238\/gmr18258"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/rbz4720170226"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.202106.016"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2006.01532.x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016-12060"},{"key":"e_1_2_11_12_1","doi-asserted-by":"crossref","unstructured":"Kovalchuk S. A.Arkhipova and\u0410.Tagmazian.2019.\u201cDevelopment of the Real\u2010Time PCR Method for Detection of ss52050737 Polymorphism of lhcgr Gene in Cattle.\u201dPaper presented at the International Scientific and Practical Conference \u201cDigital Agriculture\u2010Development Strategy\u201d ISPC 2019.","DOI":"10.2991\/ispc-19.2019.78"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/vro2.44"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2023-012"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.13057\/biodiv\/d240170"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-024-04393-1"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.33899\/ijvs.2020.126886.1407"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(00)74919-8"},{"issue":"2","key":"e_1_2_11_19_1","first-page":"220","article-title":"Estimation of Genetic Parameters for Age at First Calving and Calving Interval in Blanco Orejinegro (BON) Breed Cattle Populations in Colombia","volume":"25","author":"Rocha J. F. M.","year":"2012","journal-title":"Revista Colombiana de Ciencias Pecuarias"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.13057\/biodiv\/d240544"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2012-5784"},{"key":"e_1_2_11_22_1","first-page":"1569","article-title":"Estimates of Heritability, Repeatability and Correlation of Some Productive and Reproductive Traits of Holstein Cows in Egypt","volume":"56","author":"Somida R. A. M.","year":"2025","journal-title":"Egyptian Journal of Veterinary Science"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0297657"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731108002322"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12711-021-00650-1"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-1891-3-35"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70212","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70212","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70212","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T11:39:07Z","timestamp":1779968347000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,30]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/rda.70212"],"URL":"https:\/\/doi.org\/10.1111\/rda.70212","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,30]]},"assertion":[{"value":"2025-02-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70212"}}