{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T12:07:50Z","timestamp":1779970070111,"version":"3.53.1"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["VEGA 1\/0680\/22"],"award-info":[{"award-number":["VEGA 1\/0680\/22"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["RSP2026R17"],"award-info":[{"award-number":["RSP2026R17"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The aim of the present study was to examine the role of miR\u2010105\u20101, miR\u2010152, vinculin (VCL) and \u03b1\u2010parvin (PARVA) in the control of ovarian cell functions, and whether miRNAs and siRNAs could enter and affect cultured cells with and without the application of carriers. For this purpose, we (1) examined the incorporation, expression and effect of the selected miRNAs (miR\u2010105\u20101 and miR\u2010152 mimics and antagonists) and siRNAs downregulating VCL and PARVA on cultured porcine ovarian granulosa cells; and (2) compared the incorporation, expression, and effects of these miRNAs and siRNAs used without and with carriers (transfection reagent: Lipofectamine). The incorporation and expression of these RNAs, viability, proliferation, apoptosis and steroid hormone release of cells were analysed by immunocytochemistry, RT\u2013qPCR, the alamarBlue test, BrdU incorporation, a DNA fragmentation (Cell Death Detection) kit and ELISA. The obtained results demonstrate (1) that miR\u2010105\u20101, miR\u2010152, VCL and PARVA can be stimulators of the main basic ovarian cell functions; (2) that miRNAs can be incorporated and expressed in ovarian cells and affect their functions without a carrier; and (3) that a carrier is not necessary for siRNA incorporation and expression but is necessary for their influence on ovarian cell functions.<\/jats:p>","DOI":"10.1111\/rda.70219","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T12:41:04Z","timestamp":1778762464000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Involvement of\n                    <scp>miR<\/scp>\n                    \u2010105\u20101,\n                    <scp>miR<\/scp>\n                    \u2010152, Vinculin and \u03b1\u2010Parvin in the Control of Porcine Ovarian Cell Functions. Do\n                    <scp>miRNAs<\/scp>\n                    and\n                    <scp>siRNAs<\/scp>\n                    Need a Transfection Reagent?"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9364-3512","authenticated-orcid":false,"given":"Alexander V.","family":"Sirotkin","sequence":"first","affiliation":[{"name":"Faculty of Natural Sciences Constantine the Philosopher University in Nitra  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Fabov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences Constantine the Philosopher University in Nitra  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbora","family":"Loncov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences Constantine the Philosopher University in Nitra  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladim\u00edra","family":"Mondo\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences Constantine the Philosopher University in Nitra  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radoslav","family":"Omelka","sequence":"additional","affiliation":[{"name":"Faculty of Natural Sciences Constantine the Philosopher University in Nitra  Nitra Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2170-1303","authenticated-orcid":false,"given":"Abdel Halim","family":"Harrath","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science King Saud University  Riyadh Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,14]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2023.1052731"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2021.174178"},{"issue":"1","key":"e_1_2_13_4_1","first-page":"45","article-title":"Vinculin in Cell\u2013Cell and Cell\u2013Matrix Adhesions","volume":"339","author":"Bays J. 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