{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:00:33Z","timestamp":1790838033192,"version":"4.1.0"},"reference-count":11,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2020\u2010116531RB\u2010I00"],"award-info":[{"award-number":["PID2020\u2010116531RB\u2010I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2023\u2010149027OB\u2010I00"],"award-info":[{"award-number":["PID2023\u2010149027OB\u2010I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PRE2021\u2010098675"],"award-info":[{"award-number":["PRE2021\u2010098675"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003030","name":"Ag\u00e8ncia de Gesti\u00f3 d'Ajuts Universitaris i de Recerca","doi-asserted-by":"publisher","award":["2021SGR00900"],"award-info":[{"award-number":["2021SGR00900"]}],"id":[{"id":"10.13039\/501100003030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In vitro culture (IVC) exposes oocytes to suboptimal conditions that may disrupt redox homeostasis and compromise developmental competence. Extracellular vesicles (EVs) mediate intercellular communication, and their cargo reflects the physiological state of donor cells. We hypothesized that EVs derived from heat\u2010stressed (HS) or oxidative\u2010stressed (OS) granulosa cells (GCs) differentially modulate oocyte redox status and embryo development. GCs were exposed to 42\u00b0C for 24\u2009h or to 5\u2009\u03bcM H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    for 40\u2009min, and EVs were subsequently isolated from conditioned media. GC\u2010EVs (1\u2009\u00d7\u200910\n                    <jats:sup>9<\/jats:sup>\n                    particles\/mL) were supplemented during in\u00a0vitro maturation (IVM). Redox parameters were assessed using triple fluorescence staining for reactive oxygen species (ROS), glutathione (GSH) and mitochondrial activity, combined with 3D confocal segmentation. EV\u2010treated oocytes showed reduced ROS\/GSH ratios compared with controls, indicating an improved redox balance. HS\u2010EVs specifically enhanced mitochondrial activity. Cleavage rates were unaffected, whereas OS\u2010EVs increased Day 7 blastocyst rates and HS\u2010EVs improved hatching rates. These findings demonstrate that stress\u2010conditioned GCs\u2010EVs differentially regulate oocyte redox status, mitochondrial activity and developmental competence, supporting their potential to optimize IVC systems.\n                  <\/jats:p>","DOI":"10.1111\/rda.70224","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:00:57Z","timestamp":1790827257000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extracellular Vesicles From Heat\u2010 and Oxidative\u2010Stressed Granulosa Cells Improve Redox Balance and Embryo Development in Bovine Oocytes"],"prefix":"10.1111","volume":"61","author":[{"given":"Judith","family":"Diaz\u2010Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Autonomous University of Barcelona  Cerdanyola del Vall\u00e8s Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulia N.","family":"Cajas","sequence":"additional","affiliation":[{"name":"National Institute for Agriculture and Food Research and Technology  Madrid Spain"},{"name":"Technical University of Loja  Loja Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Ca\u00f1\u00f3n\u2010Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"National Institute for Agriculture and Food Research and Technology  Madrid Spain"},{"name":"Corporaci\u00f3n Universitaria del Huila (CORHUILA), Grupo Kyron  Huila Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Gago","sequence":"additional","affiliation":[{"name":"Autonomous University of Barcelona  Cerdanyola del Vall\u00e8s Spain"},{"name":"Embryotools R&amp;D Centre  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Olegario","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"SERIDA  Gij\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manel","family":"L\u00f3pez\u2010B\u00e9jar","sequence":"additional","affiliation":[{"name":"Autonomous University of Barcelona  Cerdanyola del Vall\u00e8s Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6813-3940","authenticated-orcid":false,"given":"Dimitrios","family":"Rizos","sequence":"additional","affiliation":[{"name":"National Institute for Agriculture and Food Research and Technology  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6733-1328","authenticated-orcid":false,"given":"Teresa","family":"Mogas","sequence":"additional","affiliation":[{"name":"Autonomous University of Barcelona  Cerdanyola del Vall\u00e8s Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11030477"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9912281"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.21451\/1984\u20103143\u2010AR2018\u20100101"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0179451"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201017981\u20104"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201072706\u2010z"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioaf096"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1142629"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0187569"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2025.1703475"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2025.103614"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70224","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70224","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70224","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:45:49Z","timestamp":1790837149000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70224"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":11,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/rda.70224"],"URL":"https:\/\/doi.org\/10.1111\/rda.70224","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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