{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:05:55Z","timestamp":1790838355135,"version":"4.1.0"},"reference-count":8,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014181","name":"Junta de Extremadura","doi-asserted-by":"publisher","award":["GR24163"],"award-info":[{"award-number":["GR24163"]}],"id":[{"id":"10.13039\/501100014181","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The acrosome reaction (AR) is a calcium\u2010dependent exocytotic event required for successful fertilization. Calcium ionophores are commonly used to induce AR in\u00a0vitro; however, this treatment is associated with a significant loss of sperm motility. The aim of this study was to evaluate the recovery of motility in boar spermatozoa after ionophore\u2010induced AR and to assess the role of extracellular calcium. Boar spermatozoa were incubated with 10\u2009\u03bcM 4\u2010Br\u2010A23187 to induce AR. Then, spermatozoa were washed and incubated for 30\u2009min in a recovery medium containing either 1\u2009mM calcium or 5\u2009mM EGTA. The AR was analysed by flow cytometry, and motility parameters were assessed using a CASA system. Ionophore treatment induced AR and caused a marked reduction in sperm motility, which was partially recovered in the presence of 5\u2009mM EGTA, but not in the presence of 1\u2009mM calcium. These results suggest that extracellular calcium inhibits the restoration of motility after ionophore\u2010induced AR in boar spermatozoa.<\/jats:p>","DOI":"10.1111\/rda.70236","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:05:11Z","timestamp":1790827511000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Restoration of Motility in Acrosome\u2010Reacted Boar Spermatozoa Induced by the Ionophore 4\u2010Br\u2010\n                    <scp>A23187<\/scp>"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6787-0006","authenticated-orcid":false,"given":"David","family":"Mart\u00edn\u2010Hidalgo","sequence":"first","affiliation":[{"name":"SINTREP Group, Departamento de Fisiolog\u00eda, Facultad de Medicina y Ciencias de la Salud Universidad de Extremadura  Badajoz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6399-1081","authenticated-orcid":false,"given":"Violeta","family":"Calle\u2010Guisado","sequence":"additional","affiliation":[{"name":"BD Group, Departamento de Anatom\u00eda, Biolog\u00eda Celular y Zoolog\u00eda, Facultad de Medicina y Ciencias de la Salud Universidad de Extremadura  Badajoz Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5331-9793","authenticated-orcid":false,"given":"Beatriz","family":"Mac\u00edas\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-548X","authenticated-orcid":false,"given":"Lauro","family":"Gonz\u00e1lez\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"SINTREP Group, Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.682790"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2016.02.006"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD17549"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.04.006"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2021-075"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biosystems.2021.104524"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.08.009"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1317113110"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70236","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70236","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70236","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:46:29Z","timestamp":1790837189000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70236"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":8,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/rda.70236"],"URL":"https:\/\/doi.org\/10.1111\/rda.70236","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-03-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70236"}}