{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T05:45:24Z","timestamp":1782711924942,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012144","name":"Hebei Agricultural University","doi-asserted-by":"publisher","award":["YJ2025030"],"award-info":[{"award-number":["YJ2025030"]}],"id":[{"id":"10.13039\/501100012144","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32372877"],"award-info":[{"award-number":["32372877"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hormonal stimulation was performed on Japanese black prepubertal calves (8\u2010 and 12\u2010month\u2010old) and heifers (2\u20133\u2010year\u2010old), followed by oocyte retrieval by ovum pick\u2010up (OPU) and in\u00a0vitro fertilization (IVF). Both calves and heifers underwent (i) follicle ablation 36 to 48\u2009h prior to follicle\u2010stimulating hormone (FSH) priming, (ii) hormonal stimulation with 100\u2009IU (calves) or 200\u2009IU (heifers) of FSH twice per day (12\u2009h interval) for 2\u2009days, and (iii) OPU following a coasting period of 40 to 42\u2009h after the last FSH injection. Similar number of calves and heifers served as control (without FSH stimulation) at each session, to their FSH primed counterpart. FSH priming significantly increased the numbers of total antral follicles (calves, 23.5\u201323.7 vs. 8.6\u20139.9; heifers, 19.5 vs. 5.4, respectively,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) and medium and large follicles compared to control animals. Embryo development of oocytes derived from calves post IVF was significantly increased by FSH stimulation, at cleavage (63.1%\u201366.1% vs. 41.0%\u201345.1%), blastocyst development (12.7%\u201313.2% vs. 1.5%\u20132.1%), and blastocyst yield per OPU per animal (1.4\u20131.5 vs. 0.1\u20130.2 embryos, respectively,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Pregnancy rate from transferred blastocysts derived from FSH primed donor calves was significantly improved to 38.6% compared to untreated donors (38.6% vs. 13.3%, respectively;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). FSH priming of 8\u2010 and 12\u2010month\u2010old Japanese black prepubertal calves prompted a similar blastocyst development (13.2% vs. 12.7%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05) of OPU oocytes post IVF. Although FSH priming was equally effective in prepubertal calves and heifers in increasing antral follicle number and recoverable oocytes, on the contrary IVF outcomes, blastocyst yields and pregnancy, were still lower when compared to heifers. In conclusion, OPU\u2010IVF can be applied to FSH\u2010primed prepubertal calves for rapid enhancement of Japanese black cattle characterised by extremely high genetic merits, by significantly reducing generation interval.\n                  <\/jats:p>","DOI":"10.1111\/rda.70242","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T08:26:40Z","timestamp":1780734400000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Beneficial Effect of Follicle\u2010Stimulating Hormone Priming on\n                    <scp>OPU<\/scp>\n                    \u2010\n                    <scp>IVF<\/scp>\n                    in Prepubertal Calves of Japanese Black Cattle (Wagyu)"],"prefix":"10.1111","volume":"61","author":[{"given":"Zhihui","family":"Liu","sequence":"first","affiliation":[{"name":"Hebei Technology Innovation Center of Cattle and Sheep Embryo, College of Animal Science and Technology Hebei Agricultural University  Baoding P.R. China"},{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences Nanjing Normal University  Nanjing P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqiu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Hebei Provincial Animal Husbandry Station  Shijiazhuang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences Nanjing Normal University  Nanjing P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyong","family":"Xu","sequence":"additional","affiliation":[{"name":"Qingdao Longmingniu Embryo Biotechnologies Co. Ltd  Qingdao P.R. 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