{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:05:44Z","timestamp":1790838344893,"version":"4.1.0"},"reference-count":7,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003741","name":"Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003741","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["31003\/2021\u20100"],"award-info":[{"award-number":["31003\/2021\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307678\/2022\u20109"],"award-info":[{"award-number":["307678\/2022\u20109"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ultrasound has attracted interest in cryobiology for its potential to enhance heat transfer and reduce thermal gradients during thawing. Little is, however, known about how ultrasound exposure affects sperm cells. This study evaluated the effects of ultrasound on the functional parameters of pig semen. Semen samples from 10 boars were diluted, packaged in 0.5\u2010mL straws at a standard freezing concentration, and exposed to ultrasound (40\u2009kHz) for 0, 15, 30, 60 or 120\u2009s. After treatment (10\u2009min), sperm motility was assessed using computer\u2010assisted sperm analysis, and plasma membrane integrity, mitochondrial membrane potential and intracellular reactive oxygen species were evaluated by flow cytometry. While short ultrasound exposure (15\u2009s) did not affect sperm functionality, longer exposure (30\u2013120\u2009s) reduced both progressive and total motility and decreased plasma membrane integrity (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Mitochondrial membrane potential remained unchanged among treatments. These results indicate that ultrasound exposure has time\u2010dependent effects on sperm function and survival, providing new insights into the potential use of ultrasound\u2010assisted strategies for thawing cryopreserved sperm.\n                  <\/jats:p>","DOI":"10.1111\/rda.70246","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:19:02Z","timestamp":1790828342000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrasound Exposure May Detrimentally Affect Sperm Function and Survival: Possible Implications for Cryobiology"],"prefix":"10.1111","volume":"61","author":[{"given":"Carine Dahl","family":"Corcini","sequence":"first","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology University of Girona  Girona Spain"},{"name":"Unit of Cell Biology, Department of Biology, Faculty of Sciences University of Girona  Girona Spain"},{"name":"Comparative Animal Reproduction, Department of Animal Pathology, College of Veterinary Medicine Federal University of Pelotas  Pelotas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Sergio","family":"Varela Junior","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology University of Girona  Girona Spain"},{"name":"Unit of Cell Biology, Department of Biology, Faculty of Sciences University of Girona  Girona Spain"},{"name":"Comparative Animal Reproduction, Institute of Biological Sciences Federal University of Rio Grande  Rio Grande Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgan Yuri Oliveira Teles","family":"Machado","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology University of Girona  Girona Spain"},{"name":"Unit of Cell Biology, Department of Biology, Faculty of Sciences University of Girona  Girona Spain"},{"name":"Comparative Animal Reproduction, Department of Animal Pathology, College of Veterinary Medicine Federal University of Pelotas  Pelotas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiane","family":"da Motta Xavier","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology University of Girona  Girona Spain"},{"name":"Unit of Cell Biology, Department of Biology, Faculty of Sciences University of Girona  Girona Spain"},{"name":"Comparative Animal Reproduction, Department of Animal Pathology, College of Veterinary Medicine Federal University of Pelotas  Pelotas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2209-340X","authenticated-orcid":false,"given":"Marc","family":"Yeste","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology University of Girona  Girona Spain"},{"name":"Unit of Cell Biology, Department of Biology, Faculty of Sciences University of Girona  Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3906\/vet\u20101507\u201018"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.05.003"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods14193446"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods15020328"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adk2864"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12569"},{"issue":"3","key":"e_1_2_11_8_1","first-page":"1","article-title":"Perspectives in Porcine Semen Conservation (Liquid and Frozen)","volume":"72","author":"Yeste M.","year":"2016","journal-title":"Journal of Reproduction and Development"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70246","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70246","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70246","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:45:48Z","timestamp":1790837148000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70246"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":7,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/rda.70246"],"URL":"https:\/\/doi.org\/10.1111\/rda.70246","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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