{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:02:56Z","timestamp":1790838176880,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["AEI\/10.13039\/501100011033"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["Project PID2022\u2010137645OB\u2010I00"],"award-info":[{"award-number":["Project PID2022\u2010137645OB\u2010I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n S\u00e9neca","doi-asserted-by":"publisher","award":["FSRM\/10.13039\/100007801"],"award-info":[{"award-number":["FSRM\/10.13039\/100007801"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003945","name":"Link\u00f6pings Universitet","doi-asserted-by":"publisher","award":["Project LiU\u2010303668"],"award-info":[{"award-number":["Project LiU\u2010303668"]}],"id":[{"id":"10.13039\/501100003945","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study explores alternative freezing approaches aimed at reducing or avoiding the use of liquid nitrogen (LN\n                    <jats:sub>2<\/jats:sub>\n                    ) to store boar semen. Sperm\u2010rich fractions from three boars were pooled and frozen in 0.25\u2010 and 0.5\u2010mL straws using four different protocols: a conventional method (Control; LN\n                    <jats:sub>2<\/jats:sub>\n                    vapours followed by immersion and storage in LN\n                    <jats:sub>2<\/jats:sub>\n                    ), FT1 (as Control but stored at \u221280\u00b0C), FT2 (LN\n                    <jats:sub>2<\/jats:sub>\n                    vapours followed by storage at \u221280\u00b0C), and FT3 (direct transfer from 5\u00b0C to \u221280\u00b0C plus storage at this temperature). Sperm quality and intracellular H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    production were evaluated at 30\u2010 and 150\u2010min after thawing. Sperm motility and viability in FT1 and FT2 were similar to Control, whereas FT3 showed poorer sperm quality and higher intracellular H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    generation. Straw size also influenced cryosurvival, being lowest in 0.5\u2009mL straws. In addition, sperm quality deteriorated alongside post\u2010thaw incubation time. In contrast, storage time (one week vs. one month) did not show a clear effect on the evaluated parameters. In conclusion, these findings suggest that freezing protocols based on LN\n                    <jats:sub>2<\/jats:sub>\n                    vapour cooling, combined with storage at \u221280\u00b0C, could be a practical alternative for short\u2010term storage of boar semen, reducing LN\n                    <jats:sub>2<\/jats:sub>\n                    use.\n                  <\/jats:p>","DOI":"10.1111\/rda.70248","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:02:56Z","timestamp":1790827376000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Frozen Boar Semen Can Be Successfully Stored at \u221280\u00b0C, Reducing Need for\n                    <scp>\n                      LN\n                      <sub>2<\/sub>\n                    <\/scp>\n                    Storage Containers"],"prefix":"10.1111","volume":"61","author":[{"given":"A.","family":"Lopez\u2010Jara","sequence":"first","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u201cCampus Mare Nostrum\u201d University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Toptsikioti","sequence":"additional","affiliation":[{"name":"Department of Agriculture, School of Geotechnical Sciences International Hellenic University  Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9317-0188","authenticated-orcid":false,"given":"M.","family":"Garcia\u2010Canovas","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u201cCampus Mare Nostrum\u201d University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cuello","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u201cCampus Mare Nostrum\u201d University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6955-7750","authenticated-orcid":false,"given":"M. A.","family":"Gil","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u201cCampus Mare Nostrum\u201d University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5194-2124","authenticated-orcid":false,"given":"H.","family":"Rodriguez\u2010Martinez","sequence":"additional","affiliation":[{"name":"Department of Biomedical &amp; Clinical Sciences (BKV), BKH\/Obstetrics &amp; Gynaecology, Faculty of Medicine and Health Sciences Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1260-9721","authenticated-orcid":false,"given":"E. A.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u201cCampus Mare Nostrum\u201d University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Parrilla","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u201cCampus Mare Nostrum\u201d University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.10371\/RD03089"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.12.024"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/BF03546946"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox13030322"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20104320(99)00035\u20104"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1530\/biosciprocs.15.0015"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ANIREPROSCI.2006.08.022"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20104939\u20101862\u20101_19"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1439\u20100531.2011.01822.X"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.THERIOGENOLOGY.2017.10.035"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.THERIOGENOLOGY.2019.04.001"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/AJPCELL.1984.247.3.C125"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2011.12.002"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20104406\u20105_13"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201036624\u20105"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.THERIOGENOLOGY.2017.05.007"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.06.020"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/MRD.22840"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70248","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70248","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70248","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:45:59Z","timestamp":1790837159000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":18,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/rda.70248"],"URL":"https:\/\/doi.org\/10.1111\/rda.70248","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70248"}}