{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T05:45:25Z","timestamp":1782711925146,"version":"3.54.5"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["305420\/2013\u20105"],"award-info":[{"award-number":["305420\/2013\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Testicular cryopreservation is a promising tool for fertility preservation, with applications in endangered species, prepubertal animals and individuals of high genetic value. This study evaluated proliferative potential and collagen deposition in adult canine testicular fragments subjected to vitrification using different warming temperatures (37\u00b0C for 1\u2009min and 50\u00b0C for 5\u2009s) and cryoprotectant combinations: ethylene glycol\/glycerol (EG\/GLY), dimethyl sulfoxide\/glycerol (DMSO\/GLY) and dimethyl sulfoxide\/ethylene glycol (DMSO\/EG). Twenty\u2010four testicular fragments (3\u2009\u00d7\u20091\u2009\u00d7\u20091\u2009mm) per dog were collected from 10 adult dogs, vitrified using the needle immersion method and subsequently warmed according to the experimental protocol. Proliferative potential was analysed using AgNOR staining, and collagen deposition was assessed using Picrosirius staining. AgNOR analysis indicated that proliferative potential was maintained across all groups, with no significant differences (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05), ranging from 2.42\u2009\u00b1\u20090.05 (EG\/GLY37) to 2.71\u2009\u00b1\u20090.31 (DMSO\/EG50). Picrosirius staining revealed increased collagen only in the DMSO\/GLY37 group (194.80\u2009\u00b1\u200917.14,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), with a significant increase in type III collagen fibres (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). All other groups maintained collagen levels similar to those of the control group (171.20\u2009\u00b1\u200918.80,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). In conclusion, the tested cryoprotectant combinations and warming temperatures adequately preserved proliferative potential, and collagen organization in vitrified adult canine testicular fragments, except DMSO\/GLY37, supporting their application in assisted reproductive technologies &amp; species conservation programmes.\n                  <\/jats:p>","DOI":"10.1111\/rda.70253","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T17:14:38Z","timestamp":1781802878000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Proliferative Potential and Collagen Deposition in Vitrified Canine Testicular Fragments Subjected to Different Cryoprotectant Combinations and Warming Temperatures"],"prefix":"10.1111","volume":"61","author":[{"given":"Juliana de Souza","family":"Fernandes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o de Carn\u00edvoros Universidade Estadual do Cear\u00e1  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssyka Ara\u00fajo","family":"Noronha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o de Carn\u00edvoros Universidade Estadual do Cear\u00e1  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5190-024X","authenticated-orcid":false,"given":"Bruna Farias","family":"Brito","sequence":"additional","affiliation":[{"name":"Centro Universit\u00e1rio Fametro  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisele Karla Sena","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o de Carn\u00edvoros Universidade Estadual do Cear\u00e1  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaby Judith Quispe","family":"Palomino","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Manipula\u00e7\u00e3o de O\u00f3citos e Fol\u00edculos Ovarianos Pr\u00e9\u2010Antrais Universidade Estadual do Cear\u00e1  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen Patricia Huayhua","family":"Llacho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Manipula\u00e7\u00e3o de O\u00f3citos e Fol\u00edculos Ovarianos Pr\u00e9\u2010Antrais Universidade Estadual do Cear\u00e1  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9793-1968","authenticated-orcid":false,"given":"L\u00facia Daniel Machado","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Reprodu\u00e7\u00e3o de Carn\u00edvoros Universidade Estadual do Cear\u00e1  Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12463"},{"issue":"1","key":"e_1_2_11_3_1","first-page":"1","article-title":"Caracteriza\u00e7\u00e3o de col\u00e1genos tipos I e III no estroma do carcinoma de c\u00e9lulas escamosas cut\u00e2neo em c\u00e3es","volume":"68","author":"Bedoya S. 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