{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:00:33Z","timestamp":1790838033072,"version":"4.1.0"},"reference-count":11,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["PID2020\u2010116090RB\u2010I00"],"award-info":[{"award-number":["PID2020\u2010116090RB\u2010I00"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Finance Code 001"],"award-info":[{"award-number":["Finance Code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This study evaluated the effect of disaccharide supplementation on zebrafish sperm stored at 5\u00b0C for 24\u2009h. Seven pooled replicates from 60 broodfish were used. Semen samples were initially cooled and, after 1\u2009h, supplemented with either HBSS (E\u2010C), Sucrose 0.13M (E\u2010S13), Trehalose 0.13M (E\u2010T13) or Trehalose 0.26M (E\u2010T26). Sperm motility (computer\u2010assisted sperm analysis) and plasma membrane integrity (flow cytometry) were evaluated over 24\u2009h of cool storage. During the first 2\u2009h, E\u2010T26 decreased sperm motility values. After 24\u2009h, E\u2010S13 and E\u2010T13 showed higher motility than E\u2010C and E\u2010T26. In conclusion, sucrose and trehalose supplementation improved sperm motility after 24\u2009h of cold storage. Further studies are needed to assess the effect of disaccharide supplementation after shorter storage periods.<\/jats:p>","DOI":"10.1111\/rda.70256","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:23:45Z","timestamp":1790828625000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Sucrose and Trehalose Supplementation on Zebrafish (\n                    <i>Danio rerio<\/i>\n                    ) Sperm Quality During Cold Storage"],"prefix":"10.1111","volume":"61","author":[{"given":"Blenda R. N.","family":"Vilela","sequence":"first","affiliation":[{"name":"Veterinary Reproduction Group (AGR\u2010275), Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine Universidad de C\u00f3rdoba  C\u00f3rdoba Spain"},{"name":"Department of Veterinary Medicine Federal University of Lavras  Lavras Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5199-6981","authenticated-orcid":false,"given":"Pilar","family":"Vallejo\u2010Soto","sequence":"additional","affiliation":[{"name":"Veterinary Reproduction Group (AGR\u2010275), Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine Universidad de C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Montero\u2010Palma","sequence":"additional","affiliation":[{"name":"Veterinary Reproduction Group (AGR\u2010275), Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine Universidad de C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Veterinary Reproduction Group (AGR\u2010275), Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine Universidad de C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nah\u00fam","family":"Ayala","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine Universidad de C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosario","family":"Moyano","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Comparative Pathology and Toxicology, Faculty of Veterinary Medicine Universidad de C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis D. S.","family":"Murgas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine Federal University of Lavras  Lavras Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2479-498X","authenticated-orcid":false,"given":"Isabel","family":"Ortiz","sequence":"additional","affiliation":[{"name":"Veterinary Reproduction Group (AGR\u2010275), Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine Universidad de C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Dorado","sequence":"additional","affiliation":[{"name":"Veterinary Reproduction Group (AGR\u2010275), Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine Universidad de C\u00f3rdoba  C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.749735"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani11061558"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12387"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/1984\u20103143\u2010AR2024\u20100075"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10695\u2010018\u20100500\u20106"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10010036"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2020.1942"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/zeb.2015.1176"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2024.08.008"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2024.741623"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.46989\/001c.20841"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70256","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70256","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70256","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:46:31Z","timestamp":1790837191000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":11,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/rda.70256"],"URL":"https:\/\/doi.org\/10.1111\/rda.70256","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70256"}}