{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:02:56Z","timestamp":1790838176936,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Understanding early embryogenesis in livestock remains a persistent bottleneck in both reproductive biology and animal breeding. Work in domestic species has long been limited by restricted access to embryos, ethical considerations, and the complexity of peri\u2010implantation development, making mechanistic studies difficult. Recent advances in stem cell biology are beginning to shift this. Blastocyst\u2010like structures, or blastoids, now offer a practical way to model early development in\u00a0vitro. Blastoids capture key features of natural blastocysts, including overall structure, lineage composition, and gene expression patterns, while being far more accessible experimentally. In livestock species, especially cattle and pigs, blastoid models are starting to gain traction. Livestock blastoids provide a tractable system to study lineage specification, epiblast\u2010extraembryonic interactions, and early conceptus development. They also open the door to improving assisted reproductive technologies by enabling controlled, scalable experimentation that is not feasible with embryos alone. In this review, we summarize recent advances in the generation of livestock blastoids and discuss how these systems can be used to study early development across livestock species and to inform next\u2010generation reproductive technologies. We highlight current limitations and outline key challenges ahead, including how blastoid models can be combined with genome editing, stem cell engineering and related embryo\u2010based approaches to improve reproductive efficiency in domestic animals.<\/jats:p>","DOI":"10.1111\/rda.70262","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:05:15Z","timestamp":1790827515000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Livestock Blastoids: In\u00a0Vitro Blastocyst Models for Embryogenesis and Reproductive Technologies"],"prefix":"10.1111","volume":"61","author":[{"given":"Cristina A.","family":"Martinez\u2010Serrano","sequence":"first","affiliation":[{"name":"Department of Molecular Biology University of Texas Southwestern Medical Center  Dallas Texas USA"},{"name":"Department of Biotechnology National Institute for Agriculture and Food Research and Technology (INIA\u2010CSIC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology University of Texas Southwestern Medical Center  Dallas Texas USA"},{"name":"Hamon Center for Regenerative Science and Medicine University of Texas Southwestern Medical Center  Dallas Texas USA"},{"name":"Cecil H. and Ida Green Center for Reproductive Biology Sciences University of Texas Southwestern Medical Center  Dallas Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2600783123"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.70073"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1716161115"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-026-00872-x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-024-03858-2"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2019.05.028"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01171.x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2024.01.009"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0333-2"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2025.111014"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-8887-3_1"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0142442"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-024-00516-y"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.199901"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v13.i1.1"},{"key":"e_1_2_11_17_1","doi-asserted-by":"crossref","unstructured":"Liao Z. 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