{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:00:34Z","timestamp":1790838034149,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003945","name":"Link\u00f6pings Universitet","doi-asserted-by":"publisher","award":["LiU\u2010303668"],"award-info":[{"award-number":["LiU\u2010303668"]}],"id":[{"id":"10.13039\/501100003945","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to evaluate the effect of supplementing in\u00a0vitro culture medium of porcine zygotes with interleukin\u20104 (IL\u20104) on their developmental competence and quality. Cumulus\u2010oocyte complexes (COCs) were matured and fertilized in\u00a0vitro. The presumptive zygotes were then cultured in\u00a0vitro for 7\u2009days with different concentrations of IL\u20104 (25\u2009pg\/mL, 100\u2009pg\/mL, 25\u2009ng\/mL and 100\u2009ng\/mL) or without IL\u20104 (control). Cleavage and blastocyst formation rates were evaluated on days 2 and 7 of culture, respectively. On day 7 of IVC, some control and 25\u2009pg\/mL blastocysts were used to assess the expression of a panel of genes related to embryo quality by real\u2010time quantitative PCR (RT\u2010qPCR). The results did not show any positive effect of IL\u20104 on embryo developmental competence, regardless of its concentration. RT\u2010qPCR revealed a significant downregulation of genes involved in cell cycle regulation (\n                    <jats:italic>PCNA<\/jats:italic>\n                    ), DNA damage response and immune pathways (\n                    <jats:italic>STAT6<\/jats:italic>\n                    and\n                    <jats:italic>GATA3<\/jats:italic>\n                    ) in blastocysts treated with IL\u20104. In conclusion, supplementation of IVC medium with IL\u20104 did not improve the developmental competence of IVP embryos; however, IL\u20104 modulated the expression of stress\u2010 and immune\u2010related genes in blastocysts.\n                  <\/jats:p>","DOI":"10.1111\/rda.70263","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:05:10Z","timestamp":1790827510000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Interleukin\u20104 Supplementation During In\u00a0Vitro Culture Modulates the Expression of Stress\u2010 and Immune\u2010Related Genes During Porcine Blastocyst Development"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9317-0188","authenticated-orcid":false,"given":"Manuela","family":"Garcia\u2010Canovas","sequence":"first","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u2018Campus Mare Nostrum\u2019 University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos","family":"Torregrosa\u2010Fuentes","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u2018Campus Mare Nostrum\u2019 University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adelina","family":"Lopez\u2010Jara","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u2018Campus Mare Nostrum\u2019 University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inmaculada","family":"Parrilla","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u2018Campus Mare Nostrum\u2019 University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5194-2124","authenticated-orcid":false,"given":"Heriberto","family":"Rodriguez\u2010Martinez","sequence":"additional","affiliation":[{"name":"Department of Biomedical &amp; Clinical Sciences (BKV), BKH\/Obstetrics &amp; Gynaecology, Faculty of Medicine and Health Sciences Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1260-9721","authenticated-orcid":false,"given":"Emilio A.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u2018Campus Mare Nostrum\u2019 University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6955-7750","authenticated-orcid":false,"given":"Maria A.","family":"Gil","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u2018Campus Mare Nostrum\u2019 University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Cuello","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, International Excellence Campus for Higher Education and Research \u2018Campus Mare Nostrum\u2019 University of Murcia  Murcia Spain"},{"name":"Institute for Biomedical Research of Murcia (IMIB\u2010Pascual Parrilla)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201075442\u20106"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fanim.2022.826324"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2025.117483"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2024.107600"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.151.9.4562"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2021.03.007"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11061177"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/7.3.265"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2023\u2010094"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10112028"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcq243"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2019.01.017"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.04.007"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1703998114"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70263","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70263","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70263","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:46:16Z","timestamp":1790837176000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70263"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":15,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/rda.70263"],"URL":"https:\/\/doi.org\/10.1111\/rda.70263","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-04-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70263"}}