{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T07:37:55Z","timestamp":1792827475251,"version":"4.5.0"},"reference-count":18,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PID2023\u2010150622OA\u2010100"],"award-info":[{"award-number":["PID2023\u2010150622OA\u2010100"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Porcine in\u00a0vitro embryo production (pIVP) would have a major impact on both livestock production and biomedical research if its efficiency\u2014currently limited by suboptimal oocyte competence\u2014could be improved. This study aimed to characterize transcriptional dynamics across oocyte in\u00a0vitro maturation (IVM) and fertilization (IVF). Immature oocytes (IMO), mature oocytes (MO) and zygotes (ZY) produced in\u00a0vitro were analysed by RNA sequencing followed by differential gene expression and functional enrichment analyses. IMO showed higher abundance of zona pellucida (ZP) transcripts (\n                    <jats:italic>ZP2<\/jats:italic>\n                    ,\n                    <jats:italic>ZP3<\/jats:italic>\n                    ,\n                    <jats:italic>ZP4<\/jats:italic>\n                    ), whereas MO were enriched in genes related to cytoskeletal remodelling and calcium signalling (e.g.,\n                    <jats:italic>ITPR1<\/jats:italic>\n                    ,\n                    <jats:italic>TGM2<\/jats:italic>\n                    ), consistent with the acquisition of cytoplasmic competence. After fertilization, ZY exhibited increased representation of transcripts involved in gamete interaction, vesicle trafficking, and maternal\u2010effect complexes (e.g., I\n                    <jats:italic>ZUMO1R<\/jats:italic>\n                    ,\n                    <jats:italic>NLRP5<\/jats:italic>\n                    ,\n                    <jats:italic>PADI6<\/jats:italic>\n                    ), reflecting the transition to post\u2010fertilization regulatory support. The chain of transcriptional shifts defines the molecular progression underlying maturation and fertilization competence in the porcine in\u00a0vitro system.\n                  <\/jats:p>","DOI":"10.1111\/rda.70265","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:05:13Z","timestamp":1790827513000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic Dynamics Associated With In\u00a0Vitro Oocyte Competence in Pigs"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6891-0477","authenticated-orcid":false,"given":"D.","family":"Gasc\u00f3n\u2010Collado","sequence":"first","affiliation":[{"name":"Department of Biotechnology National Institute for Agriculture and Food Research and Technology (INIA\u2010CSIC)  Madrid Spain"},{"name":"Complutense University of Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. G.","family":"Cabrera\u2010Gomez","sequence":"additional","affiliation":[{"name":"Department of Biotechnology National Institute for Agriculture and Food Research and Technology (INIA\u2010CSIC)  Madrid Spain"},{"name":"Complutense University of Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Blanco\u2010Cueto","sequence":"additional","affiliation":[{"name":"Department of Biotechnology National Institute for Agriculture and Food Research and Technology (INIA\u2010CSIC)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5194-2124","authenticated-orcid":false,"given":"H.","family":"Rodriguez\u2010Martinez","sequence":"additional","affiliation":[{"name":"Department of Biomedical &amp; Clinical Sciences (BKV), BKH\/Obstetrics &amp; Gynecology, Faculty of Medicine and Health Sciences Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. A.","family":"Martinez\u2010Serrano","sequence":"additional","affiliation":[{"name":"Department of Biotechnology National Institute for Agriculture and Food Research and Technology (INIA\u2010CSIC)  Madrid Spain"},{"name":"Department of Biomedical &amp; Clinical Sciences (BKV), BKH\/Obstetrics &amp; Gynecology, Faculty of Medicine and Health Sciences Link\u00f6ping University  Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/Physrev.00006.2016"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13203"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13726"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fanim.2022.826324"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.863729"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2025.01.023"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201093904\u20103"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ANIREPROSCI.2024.107600"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12993"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.619868"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dey340"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24109059"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02506"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.RBMO.2013.10.016"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioz106"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xhgg.2021.100067"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/S12864\u2010025\u201011431\u20101"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70265","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70265","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70265","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T07:08:01Z","timestamp":1792825681000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70265"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":18,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/rda.70265"],"URL":"https:\/\/doi.org\/10.1111\/rda.70265","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70265"}}