{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:23:43Z","timestamp":1791638623018,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003947","name":"Universidad Nacional de La Plata","doi-asserted-by":"publisher","award":["V11\/230"],"award-info":[{"award-number":["V11\/230"]}],"id":[{"id":"10.13039\/501100003947","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003947","name":"Universidad Nacional de La Plata","doi-asserted-by":"publisher","award":["11\/V292"],"award-info":[{"award-number":["11\/V292"]}],"id":[{"id":"10.13039\/501100003947","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Reproduction can be avoided using immunocontraception. This research aims to study the effect of the anti\u2010GnRH vaccine Improvac on clinical and reproductive function in colony\u2010housed adult queens (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Felis catus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). Short\u2010hair mixed\u2010breed queens (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200912) were housed together under a 14\u2010h photoperiod. The treated group (TRT,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20099) received 0.5\u2009mL of Improvac, and the control group (CON,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093) received 0.5\u2009mL of saline solution. Queens received two doses 30\u2009days (D) apart. Animals were divided into 4 groups (G). Control queens (G1:\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093) were ovariohysterectomized at 90\u2009D. Treated queens were ovariohysterectomized at 120\u2009D (G2,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093), at 270\u2009D (G3,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093) and at 330\u2009D (G4,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093). Data were analysed with GLIMMIX. The estrus was determined by behavioural observation and vaginal cytology. All CON queens displayed signs of estrus every 12\u201320\u2009days. In the TRT group, seven queens (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20097\/9) did not display signs of estrus until the day of ovariohysterectomy (120\u2009D [\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20092], 270\u2009D [\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20092], 330\u2009D [\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093]). However, two queens (120\u2009D [\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20091], 270\u2009D [\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20091]) displayed signs of estrus after receiving the vaccine, at G1 on D10 and G2 on D45. The clinical, haematological and biochemical parameters of treated queens remained within the reference range for the species, supporting short\u2010term tolerability of the vaccine. The TRT queens developed painless, soft tissue masses in the subcutaneous tissue at the vaccination sites. The masses appeared after the first dose but were not detectable at the end of the study. No histological abnormalities were detected in the endometrium or ovaries of control queens at 90\u2009D. However, TRT animals exhibited altered follicular development, characterised by ovarian follicular cysts, while the endometrium predominantly showed atrophic changes. This vaccine is a promising alternative for reproductive control in adult queens.\n                  <\/jats:p>","DOI":"10.1111\/rda.70268","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T10:25:48Z","timestamp":1783333548000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of an Anti\u2010\n                    <scp>GnRH<\/scp>\n                    Vaccine Improvac on Estrous Activity, and Ovarian and Uterine Morphology in Adult Queens (\n                    <i>Felis catus<\/i>\n                    )"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6634-0612","authenticated-orcid":false,"given":"Washington Fernando","family":"Carrasco\u2010Sangache","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA), Facultad de Ciencias Veterinarias Universidad Nacional de La Plata  La Plata Argentina"},{"name":"Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente Universidad Estatal de Bol\u00edvar  Guaranda Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5646-876X","authenticated-orcid":false,"given":"Mar\u00eda Carla Garc\u00eda","family":"Mitacek","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA), Facultad de Ciencias Veterinarias Universidad Nacional de La Plata  La Plata Argentina"},{"name":"CONICET  CABA Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7834-7368","authenticated-orcid":false,"given":"Mar\u00eda Cecilia","family":"Stornelli","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA), Facultad de Ciencias Veterinarias Universidad Nacional de La Plata  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8271-9833","authenticated-orcid":false,"given":"Florencia","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA), Facultad de Ciencias Veterinarias Universidad Nacional de La Plata  La Plata Argentina"},{"name":"CONICET  CABA Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Jagger Segura","family":"Ochoa","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA), Facultad de Ciencias Veterinarias Universidad Nacional de La Plata  La Plata Argentina"},{"name":"Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente Universidad Estatal de Bol\u00edvar  Guaranda Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9417-9560","authenticated-orcid":false,"given":"Rodolfo Luzbel","family":"de la Sota","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA), Facultad de Ciencias Veterinarias Universidad Nacional de La Plata  La Plata Argentina"},{"name":"Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente Universidad Estatal de Bol\u00edvar  Guaranda Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7285-1814","authenticated-orcid":false,"given":"Mar\u00eda Alejandra","family":"Stornelli","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Reproducci\u00f3n Animal (INIRA), Facultad de Ciencias Veterinarias Universidad Nacional de La Plata  La Plata Argentina"},{"name":"Universidad del Chaco Austral  S\u00e1enz Pe\u00f1a 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