{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:05:55Z","timestamp":1790838355228,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UID\/00276\/2025"],"award-info":[{"award-number":["UID\/00276\/2025"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0059\/2020"],"award-info":[{"award-number":["LA\/P\/0059\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2024.13105.PEX"],"award-info":[{"award-number":["2024.13105.PEX"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["2023.04679.BD"],"award-info":[{"award-number":["2023.04679.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Equine endometrial fibrosis is a chronic and progressive equine uterine condition characterized by fibrosis that affects mares' fertility, with a wide range of fertility outcomes being reported for these mares. Therefore, assessing layer\u2010specific collagen (COL) distribution is essential for a comprehensive understanding of endometrial remodelling and its impact on equine reproductive potential. Full\u2010thickness uterine samples, including\n                    <jats:italic>stratum functionalis<\/jats:italic>\n                    (SF),\n                    <jats:italic>stratum basalis<\/jats:italic>\n                    (SB) and myometrium, were collected post\u2010mortem from healthy control mares (Category I;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200910) and those with moderate endometrial fibrosis (Category IIB;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200910). Samples were analysed using Masson's trichrome and digital imaging. Significant differences between categories were identified exclusively within the superficial SF, where category IIB displayed higher COL area fraction (%Area) and Normalized Integrated Density than healthy controls. When evaluating variations across layers, both groups exhibited a higher %Area and Normalized Integrated Density in the SB compared to the SF, followed by a significant decrease in Normalized Integrated Density towards the myometrium. Furthermore, the Mean Grey Value progressively decreased into the deeper compartments, indicating higher staining intensity, only in the group with moderate fibrosis. In conclusion, layer\u2010dependent COL deposition underscores the value of multiparametric, quantitative approaches for an objective characterization of equine endometrial fibrosis.\n                  <\/jats:p>","DOI":"10.1111\/rda.70279","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:01:55Z","timestamp":1790827315000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Layer\u2010Specific Quantitative Assessment of Collagen Distribution in Equine Uteri Using Masson's Trichrome and Digital Image Analysis: A Comparative Study"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6432-3718","authenticated-orcid":false,"given":"Marta","family":"Cerveira\u2010Pinto","sequence":"first","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine University of Lisbon  Lisbon Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS)  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina","family":"Santos","sequence":"additional","affiliation":[{"name":"Unidade Local de Sa\u00fade de S\u00e3o Jos\u00e9\u2014Servi\u00e7o de Anatomia Patol\u00f3gica  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Gomes","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine University of Lisbon  Lisbon Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS)  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rio","family":"Pinho","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine University of Lisbon  Lisbon Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS)  Lisbon Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Sz\u00f3stek\u2010Mioduchowska","sequence":"additional","affiliation":[{"name":"InLife Institute of Animal Reproduction and Food Research Polish Academy of Science  Olsztyn Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Amaral","sequence":"additional","affiliation":[{"name":"CIISA\u2014Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine University of Lisbon  Lisbon Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS)  Lisbon Portugal"},{"name":"Comprehensive Health Research Centre (CHRC)  \u00c9vora Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Bancroft's Theory and Practice of Histological Techniques","author":"Bancroft J. 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