{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:02:57Z","timestamp":1790838177012,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The aim of this study was to evaluate the effect of silver nanoparticles (AgNPs) on donkey sperm parameters and their antimicrobial efficacy on bacteria isolated in the presence of antibiotics. Nine donkey ejaculates were collected and stored in a skim\u2010milk extender at different AgNPs concentrations (0, 0.01, 0.8, 1.6 and 3.12\u2009mg\/mL) at 5\u00b0C or 17\u00b0C. Sperm parameters, including total and progressive motility (CASA), plasma membrane integrity (PMI) and reactive oxygen species (rROS) production, were evaluated at 0, 24 and 48\u2009h of storage. Additionally, bacteria isolated from donkey semen extended with antibiotics were exposed to AgNPs to determine the minimum inhibitory\/bactericidal concentrations (MIC\/MBC). Sperm motility parameters were higher at 17\u00b0C than 5\u00b0C after 48\u2009h. AgNPs showed a dose\u2010 and time\u2010dependent cytotoxic effect, with concentrations \u2265\u20090.8\u2009mg\/mL significantly reducing motility, membrane integrity and increasing rROS after 24\u2009h. AgNPs exhibited bactericidal activity at concentrations between 1.56 and \u2265\u200925\u2009mg\/mL. In conclusion, while liquid storage at 17\u00b0C is an effective strategy for preserving donkey semen, the use of AgNPs as a standalone antimicrobial is currently limited by their cytotoxicity at effective bactericidal doses.<\/jats:p>","DOI":"10.1111\/rda.70282","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:25:27Z","timestamp":1790828727000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Silver Nanoparticles in Donkey Semen Stored at 17\u00b0C: Effect on Sperm Parameters and Bacterial Growth"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3404-2137","authenticated-orcid":false,"given":"Ver\u00f3nica P\u00e9rez","family":"Aguilera","sequence":"first","affiliation":[{"name":"Centro Militar de Cr\u00eda Caballar de \u00c1vila, Cr\u00eda Caballar de Las Fuerzas Armadas  \u00c1vila 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