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This study evaluates how selective isolation and expansion of MSC decrease GC populations. Follicular aspirates\u2010derived cells were cultured up to passage four (P4) following a previously validated protocol. While freshly isolated follicular aspirates cells (FAC) displayed heterogeneous morphologies, early passages (P3\u2010P4) showed a uniform, spindle\u2010shaped phenotype compatible with MSC. Gene expression analysis by RT\u2010qPCR revealed a marked upregulation of mesenchymal and stemness\u2010associated genes (\n                    <jats:italic>FN1, THY1, FGF2<\/jats:italic>\n                    and\n                    <jats:italic>CD105<\/jats:italic>\n                    ) in P4\u2010MSC compared with FAC, confirming enrichment of the MSC population. Conversely, GC\u2010specific genes (\n                    <jats:italic>FSHR, FOXL2<\/jats:italic>\n                    and\n                    <jats:italic>CYP11A1<\/jats:italic>\n                    ) were significantly upregulated in FAC. These findings suggest that early passages efficiently promote selective MSC expansion while progressively eliminating GC.\n                  <\/jats:p>","DOI":"10.1111\/rda.70288","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:05:16Z","timestamp":1790827516000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential Gene Expression of Mesenchymal Stromal Cell and Granulosa Cell Markers in Equine Follicular Aspirates"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8576-6700","authenticated-orcid":false,"given":"Mar\u00eda del Prado","family":"Soriano\u2010Campos","sequence":"first","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6733-4834","authenticated-orcid":false,"given":"Carmen Cristina","family":"Mu\u00f1oz\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos","family":"Luis\u2010Calero","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1843-6050","authenticated-orcid":false,"given":"Marian","family":"del Olmo\u2010Ortiz","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5011-8694","authenticated-orcid":false,"given":"Alejandro","family":"Gallardo\u2010Soler","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5331-9793","authenticated-orcid":false,"given":"Beatriz","family":"Mac\u00edas\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-548X","authenticated-orcid":false,"given":"Lauro","family":"Gonz\u00e1lez\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"SINTREP Group, Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2019.7802"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967\u2010024\u201005294\u20105"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240600855905"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/508216"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010020\u201002043\u20105"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.7546\/CRABS.2025.03.06"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010024\u201004129\u20101"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom15040561"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2025.117636"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010017\u20100616\u20100"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2018.10.005"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e27996"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287\u2010018\u20100876\u20103"}],"container-title":["Reproduction 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