{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:00:33Z","timestamp":1790838033315,"version":"4.1.0"},"reference-count":10,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Sperm capacitation requires signals from the female reproductive environment, although the molecular mechanisms governing this process remain poorly understood. Estrous sheep serum (ESS) is widely used in in\u00a0vitro fertilization of small ruminants, contributing to mimic the female reproductive fluids. Among ESS components, extracellular vesicles (ESS\u2010EVs) may mediate the transfer of molecular signals to spermatozoa and support the modifications observed when sperm are incubated with ESS. To explore this possibility, ESS\u2010EVs were isolated and validated according to MISEV2023 guidelines, and their proteome was characterized and compared with the protein signature of sperm incubated with ESS. In addition, sperm were co\u2010incubated with ESS\u2010EVs, and sperm motility and mitochondrial activity were assessed. Notably, 27.1% of proteins previously reported to be upregulated in capacitated ram sperm were identified in ESS\u2010EVs, representing a significant enrichment of capacitation\u2010related factors. Functionally, ESS\u2010EVs modulated sperm kinematics, producing a motility profile comparable to that observed under capacitation conditions, while mitochondrial activity remained unchanged. Together, these findings suggest that ESS\u2010EVs may act as molecular conveyors delivering proteins that reshape sperm motility.<\/jats:p>","DOI":"10.1111\/rda.70293","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:05:13Z","timestamp":1790827513000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Estrous Sheep Serum\u2010Derived Extracellular Vesicles Deliver Proteins That Modulate Sperm Motility in Sheep"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1921-3157","authenticated-orcid":false,"given":"Clara","family":"Vidal\u2010Garcia","sequence":"first","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2180-9434","authenticated-orcid":false,"given":"Marta","family":"Neila\u2010Montero","sequence":"additional","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1035-7101","authenticated-orcid":false,"given":"Eva","family":"D\u00edaz\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5749-4345","authenticated-orcid":false,"given":"Paula","family":"B\u00f3veda","sequence":"additional","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0885-2489","authenticated-orcid":false,"given":"Carmen Mar\u00eda","family":"Picazo\u2010C\u00f3rdoba","sequence":"additional","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7975-669X","authenticated-orcid":false,"given":"Vidal","family":"Montoro","sequence":"additional","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4310-0557","authenticated-orcid":false,"given":"Olga","family":"Garc\u00eda\u2010\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"},{"name":"ETSIA, Ciudad Real (UCLM)  Ciudad Real Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3667-6518","authenticated-orcid":false,"given":"Jos\u00e9 Juli\u00e1n","family":"Garde","sequence":"additional","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6429-5157","authenticated-orcid":false,"given":"Ana Josefa","family":"Soler","sequence":"additional","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2802-1925","authenticated-orcid":false,"given":"Mar\u00eda","family":"Iniesta\u2010Cuerda","sequence":"additional","affiliation":[{"name":"Grupo SaBio IREC (UCLM\u2010CSIC\u2010JCCM), ETSIAMB  Albacete Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201017\u20100054"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioaf248"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2025.117528"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14162363"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659\u2010026\u201000689\u20108"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201011712\u20105"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.05.032"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2020.01.046"},{"issue":"3","key":"e_1_2_9_10_1","article-title":"Isolation and Characterization of Extracellular Vesicles From Sheep Reproductive Fluids [Abstract]","volume":"21","author":"Picazo\u2010C\u00f3rdoba C. 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