{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:18:32Z","timestamp":1788351512050,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BAA\u2010AGR\/28575\/2017"],"award-info":[{"award-number":["PTDC\/BAA\u2010AGR\/28575\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/BAA\u2010AGR\/28866\/2017"],"award-info":[{"award-number":["PTDC\/BAA\u2010AGR\/28866\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Evaluating the reproductive microbiome in small ruminant livestock, such as goats and sheep, is crucial for improving breeding performance, promoting animal health, and optimizing pregnancy outcomes. A healthy microbiome in the reproductive tract inhibits infections, regulates immune activity, and reduces the risk of microbial imbalances that negatively affect pregnancy. Understanding this microbiome also supports better disease control, informs advanced animal husbandry practices for goats and sheep, and contributes to global food security. This study aimed to characterize the genital microbiome of three local Portuguese goat breeds\u2014Preta de Montesinho, Serrana, and Serpentina\u2014using 16S rRNA metabarcoding. Samples were collected from 39 goats and, for comparison, seven sheep from a local breed\u2014Serra da Estrela. Microbiome differences were obtained when integrating breed and sex factors in goats. The predominant genera in both vaginal and preputial samples included\n                    <jats:italic>Fusobacterium<\/jats:italic>\n                    ,\n                    <jats:italic>Aerococcus<\/jats:italic>\n                    ,\n                    <jats:italic>Corynebacterium<\/jats:italic>\n                    ,\n                    <jats:italic>Sediminibacterium<\/jats:italic>\n                    ,\n                    <jats:italic>Campylobacter<\/jats:italic>\n                    , and\n                    <jats:italic>Ralstonia<\/jats:italic>\n                    . Significant compositional differences were observed across breed, sex, and species as indicated by Bray\u2013Curtis dissimilarity. By characterizing the caprine reproductive microbiota through metabarcoding, this study fills an important knowledge gap and establishes a baseline for future research on disease\u2010associated changes in the male and female urogenital tract microbiota of small ruminants.\n                  <\/jats:p>","DOI":"10.1111\/rda.70297","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:44:03Z","timestamp":1785761043000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the Genital Microbiota in Portuguese Native Goats"],"prefix":"10.1111","volume":"61","author":[{"given":"Nellia Felicity","family":"Nyoni","sequence":"first","affiliation":[{"name":"CIBIO, Centro de Investiga\u00e7ao Em Biodiversidade e Recursos Geneticos, InBIO Laboratorio Associado Campus de Vair\u00e3o, Universidade do Porto  Vair\u00e3o Portugal"},{"name":"BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO  Vair\u00e3o Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Duarte\u2010Coimbra","sequence":"additional","affiliation":[{"name":"CIBIO, Centro de Investiga\u00e7ao Em Biodiversidade e Recursos Geneticos, InBIO Laboratorio Associado Campus de Vair\u00e3o, Universidade do Porto  Vair\u00e3o Portugal"},{"name":"BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO  Vair\u00e3o Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Forcina","sequence":"additional","affiliation":[{"name":"CIBIO, Centro de Investiga\u00e7ao Em Biodiversidade e Recursos Geneticos, InBIO Laboratorio Associado Campus de Vair\u00e3o, Universidade do Porto  Vair\u00e3o Portugal"},{"name":"BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO  Vair\u00e3o Portugal"},{"name":"Global Change Ecology and Evolution Research Group (GloCEE), Departamento de Ciencias de la Vida; Soil Biology and Subterranean Ecosystems Research Group (GIBSES) Universidad de Alcal\u00e1  Alcal\u00e1 de Henares Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9751-6550","authenticated-orcid":false,"given":"Luc\u00eda","family":"P\u00e9rez\u2010Pardal","sequence":"additional","affiliation":[{"name":"CIBIO, Centro de Investiga\u00e7ao Em Biodiversidade e Recursos Geneticos, InBIO Laboratorio Associado Campus de Vair\u00e3o, Universidade do Porto  Vair\u00e3o Portugal"},{"name":"BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO  Vair\u00e3o Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albano","family":"Beja\u2010Pereira","sequence":"additional","affiliation":[{"name":"CIBIO, Centro de Investiga\u00e7ao Em Biodiversidade e Recursos Geneticos, InBIO Laboratorio Associado Campus de Vair\u00e3o, Universidade do Porto  Vair\u00e3o Portugal"},{"name":"BIOPOLIS Program in Genomics, Biodiversity and Land Planning, CIBIO  Vair\u00e3o Portugal"},{"name":"DGAOT, Faculty of Sciences Universidade Do Porto  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2015.00164"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442\u20109993.2001.01070.pp.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2026.108207"},{"key":"e_1_2_11_5_1","volume-title":"Protein Metabolism in Ruminants. 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