{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:19:07Z","timestamp":1788351547899,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gestational success in ruminants depends on the maintenance of a dynamic maternal\u2013foetal microenvironment, resulting from complex interactions among the conceptus, extraembryonic membranes, maternal tissues, and gestational fluids. This study performed a comparative and longitudinal proteomic characterization of allantoic and amniotic fluids throughout pregnancy in sheep, aiming to elucidate the molecular mechanisms involved in maternal\u2013foetal communication and embryonic development. Samples of allantoic and amniotic fluids aseptically collected from extraembryonic compartments after uterine opening following animal slaughter and allocated into three gestational groups according to foetal development: early gestation (0\u201350\u2009days;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096), mid gestation (50\u2013100\u2009days;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20093) and late gestation (100\u2013150\u2009days;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20095). After centrifugation and protein quantification using the Bradford method, standardized aliquots were subjected to in\u2010solution protein digestion and analysed by nano\u2010LC\u2013ESI\u2010Q\u2010TOF mass spectrometry. A total of 201 proteins were identified in the allantoic fluid and 159 proteins in the amniotic fluid, with five proteins showing significant differences across gestational stages. In the allantoic fluid, differentially abundant proteins included alpha\u20102\u2010macroglobulin, gelsolin, basement membrane\u2013specific heparan sulphate proteoglycan core protein and alpha\u2010globin chain. In the amniotic fluid, changes were observed in peroxiredoxin\u20102, mucin\u20105\u2009AC, fetuin\u2010B, cystatin domain\u2013containing protein and globin domain\u2013containing protein. Multivariate analyses revealed progressive remodelling of the foetal extraembryonic microenvironment, associated with proteolytic control, tissue remodelling, redox homeostasis, epithelial protection and metabolic processes. Collectively, these findings reinforce the active role of foetal extraembryonic fluids in gestational physiology and highlight their potential as sources of biomarkers associated with reproductive success in sheep.\n                  <\/jats:p>","DOI":"10.1111\/rda.70300","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T10:16:51Z","timestamp":1786011411000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic Dynamics of Ovine Allantoic and Amniotic Fluids During Gestation"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0528-5124","authenticated-orcid":false,"given":"Viviane Maria","family":"Codognoto","sequence":"first","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9694-0456","authenticated-orcid":false,"given":"Rog\u00e9rio Ara\u00fajo","family":"de Almeida Filho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos Gomides","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Zanin","family":"Rates","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0088-2601","authenticated-orcid":false,"given":"Fl\u00e1vio Augusto","family":"Lourencetti","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laiza Sartori","family":"de Camargo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3553-774X","authenticated-orcid":false,"given":"Claudio Willian Delfes","family":"de Camargo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0909-9414","authenticated-orcid":false,"given":"Juanita Valentina Guzm\u00e1n","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2493-1067","authenticated-orcid":false,"given":"Luan Sit\u00f3","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0333-7437","authenticated-orcid":false,"given":"Eunice","family":"Oba","sequence":"additional","affiliation":[{"name":"Department of Veterinary Surgery and Animal Reproduction, Faculty of Veterinary Medicine and Animal Science UNESP  Botucatu Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1330709"},{"issue":"9","key":"e_1_2_11_3_1","first-page":"297","article-title":"Uterine Environment and Conceptus Development in Ruminants","volume":"9","author":"Bazer F. 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