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This study evaluated whether reproductive cyclicity and follicle count affect MSC recovery from ovum pick\u2010up (OPU)\u2010derived follicular aspirates. Twenty\u2010eight OPU procedures were performed on 13 mares, and cells were cultured until passage 3. Successful MSC cultures were obtained in 15\/28 procedures (53.6%), while 13 procedures (46.4%) yielded non\u2010MSC populations. Neither reproductive status (cycling vs. anestrous) nor follicle count influenced MSC culture success. Interestingly, unsuccessful cultures showed significantly higher cell counts at passage 0 compared to successful cultures (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). Receiver operating characteristic curve analysis revealed that passage 0 cell count was an excellent predictor of culture failure (AUC\u2009=\u20090.923;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), with a threshold of \u2265\u2009700,000 cells. These findings demonstrate that OPU\u2010derived follicular aspirates represent a reliable MSC source and that high initial cell count predicts culture failure, providing a practical early screening tool for MSC production.\n                  <\/jats:p>","DOI":"10.1111\/rda.70306","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T04:02:50Z","timestamp":1790827370000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Influence of Follicle Number and Reproductive Cyclicity on Mesenchymal Stromal Cell Yield Isolated From Equine Ovarian Follicular Aspirates"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6733-4834","authenticated-orcid":false,"given":"Carmen C.","family":"Mu\u00f1oz\u2010Garc\u00eda","sequence":"first","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8576-6700","authenticated-orcid":false,"given":"Mar\u00eda","family":"del Prado Soriano\u2010Campos","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos","family":"Luis\u2010Calero","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1843-6050","authenticated-orcid":false,"given":"Marian","family":"del Olmo\u2010Ortiz","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-548X","authenticated-orcid":false,"given":"Lauro","family":"Gonz\u00e1lez\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"SINTREP Group, Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5331-9793","authenticated-orcid":false,"given":"Beatriz","family":"Mac\u00edas\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"MINVET Group, Departamento de Medicina Animal, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/biol\u20102018\u20100034"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/508216"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14625"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010024\u201004129\u20101"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph17030350"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani15081175"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2025.117636"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13577\u2010020\u201000439\u20102"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.22.06.0240"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70306","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70306","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70306","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:46:40Z","timestamp":1790837200000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":9,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/rda.70306"],"URL":"https:\/\/doi.org\/10.1111\/rda.70306","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-03-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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