{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T12:18:32Z","timestamp":1788351512027,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cryopreservation of epididymal stallion spermatozoa is a valuable tool for fertility preservation, particularly after orchiectomy. However, the potential interactions between local anaesthesia and extender composition on post\u2010thaw spermatozoal quality remain poorly understood. This study investigated the effects of intratesticular lidocaine injection and the efficacy of various extender (egg yolk [EY] vs. lecithin [LC]) and cryoprotectant (glycerol [GLY] vs. dimethyl sulfoxide [DMSO]) combinations on stallion epididymal spermatological parameters. Using a paired  factorial design, epididymal spermatozoa were collected from both lidocaine\u2010injected (right) and non\u2010injected (left) testes of eight stallions. Samples were cryopreserved in Tris\u2010based extenders supplemented with EY\u2009+\u2009GLY, EY\u2009+\u2009DMSO, LC\u2009+\u2009GLY or LC\u2009+\u2009DMSO. Spermatozoal motility (fresh, post\u2010equilibration and post\u2010thaw), viability, plasma membrane and acrosome integrity (PMAI), mitochondrial reactive oxygen species (MROS), spermatozoa malondialdehyde (MDA) and glutathione (GSH) levels were evaluated. Intratesticular lidocaine administration had no significant effect on spermatozoal motility at any evaluation stage, and no differences were detected in any biochemical or spermatologic parameters assessed post\u2010thaw (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Regarding cryopreservation media, a significant main effect of extender type was observed for spermatozoal viability (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) and MROS levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), with EY\u2010based extenders providing superior protection compared to LC\u2010based formulations. A significant Extender \u00d7 Cryoprotectant interaction was detected for GSH levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), with the EY\u2009+\u2009GLY combination yielding the highest antioxidant capacity. No significant differences were observed in MDA levels among treatment groups (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&gt;\u20090.05). Intratesticular lidocaine administration during orchiectomy does not compromise epididymal spermatozoal quality after cryopreservation. Among the evaluated cryopreservation strategies, the combination of egg yolk and glycerol in a Tris\u2010based extender provides the most effective protection against oxidative stress and membrane damage in stallion epididymal spermatozoa.\n                  <\/jats:p>","DOI":"10.1111\/rda.70307","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:11:31Z","timestamp":1786626691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Effect of Different Extenders and Testicular Anaesthesia During Orchiectomy on Post\u2010Thawed Spermatological Parameters of Stallion Epididymal Spermatozoa"],"prefix":"10.1111","volume":"61","author":[{"given":"Harun","family":"Cinar","sequence":"first","affiliation":[{"name":"Department of Surgery, Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University  Burdur Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0911-3901","authenticated-orcid":false,"given":"Mine","family":"Herdogan","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Health Science Institute Burdur Mehmet Akif Ersoy University  Burdur Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9788-3238","authenticated-orcid":false,"given":"Feyzanur","family":"Mart","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Health Science Institute Burdur Mehmet Akif Ersoy University  Burdur Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1622-2719","authenticated-orcid":false,"given":"Hasan Ali","family":"Cay","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Health Science Institute Burdur Mehmet Akif Ersoy University  Burdur Turkey"},{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Burdur Mehmet Akif University  Burdur Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim Taha","family":"Danisman","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Veterinary Medicine Burdur Mehmet Akif Ersoy University  Burdur Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6954-6309","authenticated-orcid":false,"given":"Muhammed Enes","family":"Inanc","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Burdur Mehmet Akif University  Burdur Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burcu Menekse","family":"Balkan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine Burdur Mehmet Akif University  Burdur Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3460-522X","authenticated-orcid":false,"given":"Sukru","family":"Gungor","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Burdur Mehmet Akif University  Burdur Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0590-5995","authenticated-orcid":false,"given":"Ayhan","family":"Ata","sequence":"additional","affiliation":[{"name":"Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine Burdur Mehmet Akif University  Burdur 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