{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T10:34:00Z","timestamp":1793010840943,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T00:00:00Z","timestamp":1792195200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cryopreservation of buck semen is associated with excessive reactive oxygen species generation, oxidative stress and cellular damage, leading to reduced post\u2010thaw sperm quality. This study evaluated the effects of zinc oxide nanoparticles (ZnO\u2010NPs) added to a Tris\u2013egg yolk\u2013citric acid extender on the post\u2010thaw functional, biochemical and ultrastructural characteristics of Beetal buck spermatozoa. Ten independent pooled ejaculates were equally divided among four treatment groups containing 0 (control), 0.05, 0.10 and 0.20\u2009mg\/mL ZnO\u2010NPs before cryopreservation. Post\u2010thaw sperm quality was evaluated using conventional and fluorescence\u2010based assays, including motility, plasma membrane and acrosomal integrity, viability, DNA integrity, mitochondrial membrane potential, superoxide dismutase (SOD) activity and malondialdehyde (MDA) concentration. Transmission electron microscopy (TEM) was used to assess sperm ultrastructure in the control and 0.10\u2009mg\/mL ZnO\u2010NP groups. ZnO\u2010NP supplementation significantly improved post\u2010thaw sperm motility, plasma membrane functionality, viability, acrosomal integrity, SOD activity, DNA integrity and mitochondrial membrane potential, while reducing MDA concentration compared with the control (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). Sperm abnormalities were not significantly affected by ZnO\u2010NP supplementation. Among the tested concentrations, 0.10\u2009mg\/mL ZnO\u2010NPs showed the most favourable numerical profile across the evaluated parameters. TEM further indicated better preservation of the plasma membrane, acrosome, mitochondrial sheath and axonemal organization in the 0.10\u2009mg\/mL group than in the control. Overall, ZnO\u2010NP supplementation was associated with improved post\u2010thaw functional and ultrastructural sperm quality and a more favourable oxidative status in cryopreserved Beetal buck semen.\n                  <\/jats:p>","DOI":"10.1111\/rda.70326","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T07:41:01Z","timestamp":1792222861000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Zinc Oxide Nanoparticles on Post\u2010Thaw Functional, Biochemical and Ultrastructural Characteristics of Cryopreserved Beetal Buck (\n                    <scp>\n                      <i>Capra\u00a0hircus<\/i>\n                    <\/scp>\n                    ) Spermatozoa"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3031-1874","authenticated-orcid":false,"given":"Soihem Diana","family":"Rongmei","sequence":"first","affiliation":[{"name":"Department of Animal Reproduction Gynaecology and Obstetrics College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2054-6388","authenticated-orcid":false,"given":"Mohua Das","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction Gynaecology and Obstetrics College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4042-8113","authenticated-orcid":false,"given":"Keshav","family":"Yogi","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction Gynaecology and Obstetrics College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3174-7800","authenticated-orcid":false,"given":"W. Lomen","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction Gynaecology and Obstetrics College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9500-3744","authenticated-orcid":false,"given":"Kanchan","family":"Joshi","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction Gynaecology and Obstetrics College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudip","family":"Sinha","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction Gynaecology and Obstetrics College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6791-1974","authenticated-orcid":false,"given":"Arijit","family":"Shome","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biochemistry College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9586-1342","authenticated-orcid":false,"given":"Maradona","family":"Nath","sequence":"additional","affiliation":[{"name":"AAU\u2010Goat Research Station College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2976-7493","authenticated-orcid":false,"given":"Mitali Dutta","family":"Choudhury","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction Gynaecology and Obstetrics College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arpana","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Animal Genetics and Breeding College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shantanu","family":"Tamuly","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biochemistry College of Veterinary Science, Assam Veterinary and Fishery University  Guwahati Assam India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10091508"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14193"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12011-022-03551-6"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9020111"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.46897\/livestockstudies.610106"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03268.x"},{"key":"e_1_2_12_8_1","volume-title":"Integrative Male Reproductive Health: Risk, Mechanisms, and Interventions","author":"Berean D. 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