{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T10:34:01Z","timestamp":1793010841743,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T00:00:00Z","timestamp":1791763200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cryopreservation of stallion semen remains suboptimal, with approximately one\u2010third of stallions producing semen that is poorly tolerant to freezing. As optimization of pre\u2010freezing procedures may improve post\u2010thaw sperm quality, this study evaluated the effect of different equilibration times before freezing on the post\u2010thaw quality of stallion semen. For this purpose, 21 ejaculates were initially collected from 5 stallions and 15 were included in the final analysis after predefined exclusions. These ejaculates were separated from the seminal plasma via centrifugation in INRA 96 medium. After removal of the supernatant, each ejaculate was divided into three aliquots and equilibrated at 4\u00b0C for 2, 4, or 6\u2009h before freezing. The pellets were mixed with INRA FREEZE solution at a concentration of 100\u2009million spermatozoa per millilitre. Subsequently, the semen was frozen in 0.5\u2009mL straws after being kept at the designated temperature at +4\u00b0C for either 2, 4 or 6\u2009h post\u2010cooling. Post\u2010thaw sperm total motility, progressive motility and kinematic parameters were assessed by computer\u2010assisted sperm analysis (CASA). Total motility increased with equilibration time, from 27%\u2009\u00b1\u20097% after 2\u2009h to 34%\u2009\u00b1\u200910% after 4\u2009h and 40%\u2009\u00b1\u20097% after 6\u2009h (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Progressive motility was significantly lower after 2\u2009h (15%\u2009\u00b1\u20096%) than after 4\u2009h (20%\u2009\u00b1\u20097%) and 6\u2009h (22%\u2009\u00b1\u20097%) of equilibration (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), whereas no significant difference was observed between 4 and 6\u2009h. In contrast, equilibration time did not significantly affect sperm kinematic parameters, including VAP, VSL, VCL, ALH and LIN. These findings indicate that extending the equilibration period to 6\u2009h at 4\u00b0C before freezing improves post\u2010thaw total and progressive sperm motility in stallions without altering the movement characteristics of surviving spermatozoa.\n                  <\/jats:p>","DOI":"10.1111\/rda.70327","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T13:17:01Z","timestamp":1791811021000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Different Equilibration Times in the Freezing Process on Post\u2010Thaw Motility Parameters in Equine Spermatozoa"],"prefix":"10.1111","volume":"61","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8893-5748","authenticated-orcid":false,"given":"Hamed","family":"Farnia","sequence":"first","affiliation":[{"name":"Laboratory of Biotechnology and Pathology of Reproduction ONIRIS: College of Veterinary Medicine, Food Science and Engineering  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Sautejeau\u2010Rizzoni","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Pathology of Reproduction ONIRIS: College of Veterinary Medicine, Food Science and Engineering  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mouna","family":"Chekchouk","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Pathology of Reproduction ONIRIS: College of Veterinary Medicine, Food Science and Engineering  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9dha","family":"Belala","sequence":"additional","affiliation":[{"name":"Biotechnologies Laboratory Related to Animal Reproduction (LBRA) Institute of Veterinary Medicine, Saad Dahleb Blida University 1  Blida Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0900-3908","authenticated-orcid":false,"given":"Nora","family":"Mimoune","sequence":"additional","affiliation":[{"name":"Higher National Veterinary School  Algiers Algeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Teixeira Fangueiro","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Pathology of Reproduction ONIRIS: College of Veterinary Medicine, Food Science and Engineering  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Thorin","sequence":"additional","affiliation":[{"name":"Department of Statistics ONIRIS: College of Veterinary Medicine, Food Science and Engineering  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Leborgne","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Pathology of Reproduction ONIRIS: College of Veterinary Medicine, Food Science and Engineering  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morgane","family":"Le\u2010Guiriec","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Pathology of Reproduction ONIRIS: College of Veterinary Medicine, Food Science and Engineering  Nantes France"},{"name":"Center for Research in Transplantation and Translational Immunology, UMR 1064 INSERM, Nantes Universit\u00e9, CHU Nantes  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7502-1717","authenticated-orcid":false,"given":"Djemil","family":"Bencharif","sequence":"additional","affiliation":[{"name":"Laboratory of Biotechnology and Pathology of Reproduction ONIRIS: College of Veterinary Medicine, Food Science and Engineering  Nantes France"},{"name":"Center for Research in Transplantation and Translational Immunology, UMR 1064 INSERM, Nantes Universit\u00e9, CHU Nantes  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2015.03.002"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci11020065"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cveq.2016.08.003"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani10061033"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2016.03.010"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani16010021"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/15.6.1322"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2023.104499"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022\u20100302(04)70065\u2010X"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2018.05.012"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2009.05.005"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.54680\/fr23410110412"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2009.12.029"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.5772\/34473"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1071\/AN24013"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.58292\/CT.v16.10832"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.08.005"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.59913\/dagr.2023.12254"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.04.005"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.05.028"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.10.033"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2023.104579"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2007.10.002"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2020.106315"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2006.00784.x"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.11.034"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2008.01097.x"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2020.106547"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.06.023"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13142285"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.4322\/rbcv.2014.105"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.11118\/actaun.2023.003"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093\u2010691X(02)01297\u20109"},{"key":"e_1_2_12_35_1","first-page":"1973","article-title":"R: A Language and Environment for Statistical Computing","volume":"59","author":"R Core Team","year":"2024","journal-title":"R Foundation for Statistical Computing"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.10.014"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12214"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deag005"},{"issue":"3","key":"e_1_2_12_39_1","first-page":"279","article-title":"Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus bubalis) Spermatozoa","volume":"16","author":"Shahverdi A.","year":"2014","journal-title":"Cell Journal"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12233271"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2016.02.004"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani15121691"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.5578\/kvj.54069"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/and.14154"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70327","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70327","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T09:53:42Z","timestamp":1793008422000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":43,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/rda.70327"],"URL":"https:\/\/doi.org\/10.1111\/rda.70327","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-09-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70327"}}