{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:07:19Z","timestamp":1791133639586,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T00:00:00Z","timestamp":1366848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2013,5]]},"abstract":"Abstract<\/jats:title>Background and objective<\/jats:title>CD<\/jats:styled-content>8+<\/jats:sup> cell infiltration and apoptosis of airway epithelial cells are increased in chronic obstructive pulmonary disease (COPD<\/jats:styled-content>). CD<\/jats:styled-content>8+<\/jats:sup> T<\/jats:styled-content> cells induce apoptosis by releasing granzymes, which can also cause extracellular matrix degradation and remodelling. Granzyme B<\/jats:styled-content> levels and T<\/jats:styled-content> cells expressing granzyme B<\/jats:styled-content> are increased in bronchoalveolar lavage fluid of COPD<\/jats:styled-content> patients, which suggests that granzyme B<\/jats:styled-content> may contribute to the pathogenesis of COPD<\/jats:styled-content>. This study provides quantitation of granzyme B<\/jats:styled-content>\u2010positive cells in relation to CD<\/jats:styled-content>8+<\/jats:sup> cells in the small airway walls of emphysema.<\/jats:p><\/jats:sec>Methods<\/jats:title>Antibodies against CD<\/jats:styled-content>8 and granzyme B<\/jats:styled-content> were used to identify CD<\/jats:styled-content>8+<\/jats:sup> and granzyme B<\/jats:styled-content>+<\/jats:sup> cells. Volume fraction (Vv<\/jats:styled-content>) of CD<\/jats:styled-content>8+<\/jats:sup> and granzyme B<\/jats:styled-content>+<\/jats:sup> cells were quantitated by the point counting method in the small airways of 13 non\u2010smoker control subjects and 46 emphysema patients (14 panlobular emphysema (PLE<\/jats:styled-content>) and 32 centrilobular emphysema (CLE<\/jats:styled-content>) lungs). Immunohistochemical detection of macrophage scavenger receptor was also performed in randomly selected cases.<\/jats:p><\/jats:sec>Results<\/jats:title>The Vv<\/jats:styled-content> of CD<\/jats:styled-content>8+<\/jats:sup> and granzyme B<\/jats:styled-content>+<\/jats:sup> cells in CLE<\/jats:styled-content> was greater than those in control and PLE<\/jats:styled-content> (both P<\/jats:styled-content><\/jats:italic>\u2009<\u20090.001) subjects. The Vv<\/jats:styled-content> of granzyme B<\/jats:styled-content>+<\/jats:sup> cells was greater than that of CD<\/jats:styled-content>8+<\/jats:sup> cells (P<\/jats:styled-content><\/jats:italic>\u2009=\u20090.006), and not all CD<\/jats:styled-content>8+<\/jats:sup> cells were positive for granzyme B<\/jats:styled-content> in CLE<\/jats:styled-content> subjects. Monocytes expressing both granzyme B<\/jats:styled-content> and macrophage scavenger receptor and granulocytes expressing granzyme B<\/jats:styled-content> were identified.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Upregulation of granzyme B<\/jats:styled-content> in CD<\/jats:styled-content>8+<\/jats:sup> and non\u2010CD<\/jats:styled-content>8+<\/jats:sup> cells is an early phenomenon of small airway wall remodelling in centrilobular emphysema and suggests its possible role in the pathogenesis of COPD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.12069","type":"journal-article","created":{"date-parts":[[2013,2,20]],"date-time":"2013-02-20T10:36:06Z","timestamp":1361356566000},"page":"688-696","source":"Crossref","is-referenced-by-count":38,"title":["A possible role for CD<\/scp>8+<\/sup> and non\u2010CD<\/scp>8+<\/sup> cell granzyme B<\/scp> in early small airway wall remodelling in centrilobular emphysema"],"prefix":"10.1111","volume":"18","author":[{"given":"Won\u2010Dong","family":"Kim","sequence":"first","affiliation":[{"name":"Division of Pulmonary Medicine Konkuk University Medical Center Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun\u2010Sook","family":"Chi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Asan Medical Center, University of Ulsan College of Medicine Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang\u2010Hyeon","family":"Choe","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Chungbuk National University College of Medicine Cheongju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon\u2010Mok","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Asan Medical Center, University of Ulsan College of Medicine Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang\u2010Do","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Asan Medical Center, University of Ulsan College of Medicine Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu\u2010Rae","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology Asan Medical Center, University of Ulsan College of Medicine Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang\u2010Ha","family":"Yoo","sequence":"additional","affiliation":[{"name":"Division of Pulmonary Medicine Konkuk University Medical Center Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Ngan","sequence":"additional","affiliation":[{"name":"The James Hogg iCAPTURE Center for Cardiovascular and Pulmonary Research St. Paul's Hospital University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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