{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:31:01Z","timestamp":1788827461246,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,4,25]],"date-time":"2013-04-25T00:00:00Z","timestamp":1366848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2013,5]]},"abstract":"Abstract<\/jats:title>Background and objective<\/jats:title>Anomalies in the regulation of cyclooxygenase (COX<\/jats:styled-content>)\u20101 and \u20102 have been described in nasal polyps of aspirin\u2010induced asthma (AIA<\/jats:styled-content>). Whether these anomalies are specific to nasal polyps or affect all the nasal mucosa (NM<\/jats:styled-content>) of upper airways is still unclear. The objective of this study was to compare the COX<\/jats:styled-content> pathway in NM<\/jats:styled-content> of AIA<\/jats:styled-content> patients with the NM<\/jats:styled-content> of control subjects.<\/jats:p><\/jats:sec>Methods<\/jats:title>Fibroblasts were isolated from NM<\/jats:styled-content> of five AIA<\/jats:styled-content> patients (AIA\u2010NM<\/jats:styled-content>) and five control subjects (control\u2010NM<\/jats:styled-content>). Cells were treated with 10\u2009ng\/mL interleukin (IL<\/jats:styled-content>)\u20101\u03b2 for up to 72\u2009h. Prostaglandin E2<\/jats:sub><\/jats:styled-content> (PGE2<\/jats:sub><\/jats:styled-content>) production was measured by enzyme\u2010linked immunosorbent assay (ELISA<\/jats:styled-content>), expression of COX<\/jats:styled-content>\u20101 protein by W<\/jats:styled-content>estern blot and COX<\/jats:styled-content>\u20102 protein by ELISA<\/jats:styled-content>, W<\/jats:styled-content>estern blot and immunofluorescence techniques.<\/jats:p><\/jats:sec>Results<\/jats:title>IL<\/jats:styled-content>\u20101\u03b2 increased PGE2<\/jats:sub><\/jats:styled-content> production and COX<\/jats:styled-content>\u20101 protein expression in control\u2010NM<\/jats:styled-content> fibroblasts, but no changes were found in AIA\u2010NM<\/jats:styled-content>. IL<\/jats:styled-content>\u20101\u03b2 provoked a significant time\u2010dependent increase in COX<\/jats:styled-content>\u20102 protein expression in control\u2010NM<\/jats:styled-content> fibroblasts but had a very mild effect on COX<\/jats:styled-content>\u20102 protein expression in AIA\u2010NM<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Our data suggest that abnormalities in the COX<\/jats:styled-content> pathway are not a phenomenon exclusive to nasal\u2010polyp mucosa as they are also present in all the NM<\/jats:styled-content> of AIA<\/jats:styled-content> patients. These anomalies may be involved in the pathogenesis of airway inflammation and non\u2010steroidal anti\u2010inflammatory drug intolerance in asthma patients with chronic rhinosinusitis and nasal polyposis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.12076","type":"journal-article","created":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T10:33:57Z","timestamp":1361961237000},"page":"711-717","source":"Crossref","is-referenced-by-count":34,"title":["Low prostaglandin E2<\/sub><\/scp> and cyclooxygenase expression in nasal mucosa fibroblasts of aspirin\u2010intolerant asthmatics"],"prefix":"10.1111","volume":"18","author":[{"given":"Jordi","family":"Roca\u2010Ferrer","sequence":"first","affiliation":[{"name":"Immunoal\u00b7l\u00e8rgia Respirat\u00f2ria Cl\u00ednica i Experimental Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS) Barcelona Spain"},{"name":"Centro de Investigaciones Biom\u00e9dicas en Red de Enfermedades Respiratorias (CIBERES) Barcelona 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