{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T17:40:00Z","timestamp":1774114800754,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,10,28]],"date-time":"2013-10-28T00:00:00Z","timestamp":1382918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Neutrophils are consistently found in inflamed and infected airways in idiopathic bronchiectasis, but relatively little is known about the function of blood neutrophils in this condition. We hypothesized that peripheral blood neutrophil (PBN) phagocytosis and superoxide generation are impaired in bronchiectasis, and that granulocyte\u2010macrophage colony\u2010stimulating factor (<jats:styled-content style=\"fixed-case\">GM\u2010CSF<\/jats:styled-content>) is capable of improving neutrophil function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Neutrophils were isolated from the peripheral blood of patients with idiopathic bronchiectasis who were free of exacerbation, and from healthy controls of similar age (<jats:italic>n<\/jats:italic>\u2009=\u200921 in both groups). Ingestion of serum\u2010opsonized zymosan by neutrophils was used to quantify phagocytic capacity. Superoxide generation in neutrophils was measured in response to addition of platelet activating factor and formyl\u2010methionyl\u2010leucyl\u2010phenylalanine. Experiments were performed in the presence or absence of <jats:styled-content style=\"fixed-case\">GM\u2010CSF<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No differences were observed in either phagocytic capacity (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009=\u20090.99) or superoxide generation (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009=\u20090.81) when comparing patients and controls. However, a significant increase in phagocytic capacity above baseline levels in both patients (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009&lt;\u20090.005) and controls (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009&lt;\u20090.005) was induced by <jats:styled-content style=\"fixed-case\">GM\u2010CSF<\/jats:styled-content>. Similarly, the superoxide generation in patients (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009&lt;\u20090.005) and controls (<jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><\/jats:italic>\u2009=\u20090.001) was significantly increased by <jats:styled-content style=\"fixed-case\">GM\u2010CSF<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>PBN function was preserved in idiopathic bronchiectasis. Enhancement of neutrophil phagocytosis and superoxide generation by <jats:styled-content style=\"fixed-case\">GM\u2010CSF<\/jats:styled-content> requires further study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.12138","type":"journal-article","created":{"date-parts":[[2013,6,3]],"date-time":"2013-06-03T13:12:35Z","timestamp":1370265155000},"page":"1230-1235","source":"Crossref","is-referenced-by-count":17,"title":["Effect of granulocyte\u2010macrophage colony\u2010stimulating factor on neutrophil function in idiopathic bronchiectasis"],"prefix":"10.1111","volume":"18","author":[{"given":"Marie\u2010H\u00e9l\u00e8ne","family":"Ruchaud\u2010Sparagano","sequence":"first","affiliation":[{"name":"Institute of Cellular Medicine Newcastle University  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen","family":"Gertig","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Newcastle University  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katy L.M.","family":"Hester","sequence":"additional","affiliation":[{"name":"Adult Bronchiectasis Unit Freeman Hospital  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James G.","family":"Macfarlane","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Newcastle University  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul A.","family":"Corris","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Newcastle University  Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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