{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T12:43:11Z","timestamp":1772628191933,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2013,11,4]],"date-time":"2013-11-04T00:00:00Z","timestamp":1383523200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Respiratory syncytial virus (<jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>) results in acute wheezing in infants and is frequently associated with recurrent wheezing. Although <jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>\u2010induced wheezing clinically resembles that of asthma, corticosteroids are not equivalently effective in <jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>\u2010associated wheezing. The study sought to determine the mechanisms of <jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>\u2010induced wheezing by establishing an <jats:italic>in vitro<\/jats:italic> model of <jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>\u2010infected human bronchial epithelial cells (16\u2010<jats:styled-content style=\"fixed-case\">HBEC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Leukotriene <jats:styled-content style=\"fixed-case\">C<jats:sub>4<\/jats:sub><\/jats:styled-content> synthase (<jats:styled-content style=\"fixed-case\">LTC<jats:sub>4<\/jats:sub>S<\/jats:styled-content>) messenger <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>) expression in 16\u2010<jats:styled-content style=\"fixed-case\">HBEC<\/jats:styled-content> was detected using fluorescence quantitative polymerase chain reaction, and the relative level of <jats:styled-content style=\"fixed-case\">LTC<jats:sub>4<\/jats:sub>S mRNA<\/jats:styled-content> was expressed as quotient cycle threshold (<jats:styled-content style=\"fixed-case\">qCt<\/jats:styled-content>) based on the threshold cycle number value compared with that of \u03b2\u2010actin. Cysteinyl leukotrienes (<jats:styled-content style=\"fixed-case\">CysLT<\/jats:styled-content>) in culture supernatant were measured by enzyme\u2010linked immunosorbent assay. <jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>\u2010infected 16\u2010<jats:styled-content style=\"fixed-case\">HBEC<\/jats:styled-content> was incubated with gradient concentration of budesonide (<jats:styled-content style=\"fixed-case\">BUD<\/jats:styled-content>) to assess its effects on <jats:styled-content style=\"fixed-case\">LTC<jats:sub>4<\/jats:sub>S<\/jats:styled-content> expression and <jats:styled-content style=\"fixed-case\">CysLT<\/jats:styled-content> secretion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content> infection resulted in increased <jats:styled-content style=\"fixed-case\">LTC<jats:sub>4<\/jats:sub>S mRNA<\/jats:styled-content> expression between 48 and 96\u2009h post\u2010infection. High level of <jats:styled-content style=\"fixed-case\">CysLT<\/jats:styled-content> was detected in the supernatant of <jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>\u2010infected 16\u2010<jats:styled-content style=\"fixed-case\">HBEC<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">BUD<\/jats:styled-content> at concentrations of 10<jats:sup>\u221210<\/jats:sup> to 10<jats:sup>\u22125<\/jats:sup>\u2009mol\/<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content> did not significantly alter <jats:styled-content style=\"fixed-case\">LTC<jats:sub>4<\/jats:sub>S mRNA<\/jats:styled-content> expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content> infection upregulated <jats:styled-content style=\"fixed-case\">LTC<jats:sub>4<\/jats:sub>S<\/jats:styled-content> expression in <jats:styled-content style=\"fixed-case\">HBEC<\/jats:styled-content> leading to increased <jats:styled-content style=\"fixed-case\">CysLT<\/jats:styled-content> secretion. Such induction was not attenuated by <jats:styled-content style=\"fixed-case\">BUD<\/jats:styled-content>, suggesting that <jats:styled-content style=\"fixed-case\">CysLT<\/jats:styled-content> might contribute to the pathogenesis of <jats:styled-content style=\"fixed-case\">RSV<\/jats:styled-content>\u2010induced wheezing.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.12188","type":"journal-article","created":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T02:11:23Z","timestamp":1383617483000},"page":"40-46","source":"Crossref","is-referenced-by-count":7,"title":["Respiratory syncytial virus induces leukotriene <scp>C<\/scp><sub>4<\/sub> synthase expression in bronchial epithelial cells"],"prefix":"10.1111","volume":"18","author":[{"given":"Li\u2010hong","family":"Sun","sequence":"first","affiliation":[{"name":"State Key Laboratory of Respiratory Disease The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai\u2010huan","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi\u2010feng","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason J.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology &amp; Cell Biology Columbia University  New York New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010da","family":"Guan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie\u2010ling","family":"Wu","sequence":"additional","affiliation":[{"name":"Guangdong Maternal and Children's Hospital  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Qin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan\u2010shan","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease The First Affiliated Hospital of Guangzhou Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(96)70071-9"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.02033.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200110000-00016"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9537"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198810000-00018"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.23"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2001.00002.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2007.02733.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.01086.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209666"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.02.020"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(03)00062-1"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-012-6_9"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/01902140490252812"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.164.10.2103013"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00130608"},{"key":"e_1_2_7_19_1","first-page":"3","article-title":"Infantile wheezing and bronchodilator treatment","volume":"46","author":"Lin YZ","year":"2005","journal-title":"Acta Paediatr. 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