{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:37:57Z","timestamp":1762101477265},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,12,23]],"date-time":"2013-12-23T00:00:00Z","timestamp":1387756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2014,1]]},"abstract":"Abstract<\/jats:title>Background and objective<\/jats:title>While commercial liquid culture techniques have emerged over 20 years ago, Z<\/jats:styled-content>iehl\u2013N<\/jats:styled-content>eelsen (ZN<\/jats:styled-content>) smear microscopy remains the primary method for diagnosis of tuberculosis (TB<\/jats:styled-content>) in C<\/jats:styled-content>hina because of cost considerations. The microscopic observation drug susceptibility (MODS<\/jats:styled-content>) assay has been evaluated in different parts of the world to determine whether it can give comparable result to commercial liquid techniques. However, most reports detail evaluation of sputum specimens. This study evaluated the performance of MODS<\/jats:styled-content> assay for detection of M<\/jats:styled-content>ycobacterium tuberculosis<\/jats:italic> in extrapulmonary specimens in a C<\/jats:styled-content>hinese population.<\/jats:p><\/jats:sec>Methods<\/jats:title>A total of 173 samples, including pleural fluid (n<\/jats:styled-content><\/jats:italic>\u2009=\u2009112) and cerebrospinal fluid (CSF<\/jats:styled-content>, n<\/jats:styled-content><\/jats:italic>\u2009=\u200961) samples, were collected from patients suspected to have extrapulmonary TB<\/jats:styled-content> and tested by ZN<\/jats:styled-content> smear microscopy, L<\/jats:styled-content>owenstein\u2013J<\/jats:styled-content>ensen (LJ<\/jats:styled-content>) culture and the MODS<\/jats:styled-content> assay. Discordant results among MODS<\/jats:styled-content> assay and the other two methods were resolved by 90\u2010day follow\u2010up evaluation for all suspected patients.<\/jats:p><\/jats:sec>Results<\/jats:title>The sensitivity of the MODS<\/jats:styled-content> assay on pleural fluid and CSF<\/jats:styled-content> samples was 20.5% and 37.5%, respectively, while the specificity of MODS<\/jats:styled-content> assay on both types of samples approximated 100%. The median time to culture results for the MODS<\/jats:styled-content> and LJ<\/jats:styled-content> methods was 14 days, 32 days for pleural fluid, and 9 days and 31 days for CSF<\/jats:styled-content> samples, respectively.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>MODS<\/jats:styled-content> assay is useful to diagnose extrapulmonary TB<\/jats:styled-content> and may be an effective and affordable method in resource\u2010limited countries.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.12192","type":"journal-article","created":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T13:24:00Z","timestamp":1381238640000},"page":"132-137","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of the performance of the microscopic observation drug susceptibility assay for diagnosis of extrapulmonary tuberculosis in C<\/scp>hina: A preliminary study"],"prefix":"10.1111","volume":"19","author":[{"given":"Zikun","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory the First Affiliated Hospital of Nanchang University Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoliang","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Chest Hospital of Jiangxi Province Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory the First Affiliated Hospital of Nanchang University Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bixia","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory the First Affiliated Hospital of Nanchang University Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weitin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory the First Affiliated Hospital of Nanchang University Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaomeng","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory the First Affiliated Hospital of Nanchang University Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory the First Affiliated Hospital of Nanchang University Nanchang Jiangxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,12,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60359-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0907847"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001536"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00355-08"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.1.20-28.2006"},{"key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1128\/JCM.38.3.1203-1208.2000","article-title":"Rapid, efficient detection and drug susceptibility testing of M. tuberculosis in sputum by microscopic observation of broth cultures. The Tuberculosis Working Group in Peru","volume":"38","author":"Caviedes L","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_7_8_1","first-page":"217","article-title":"Reliability of the MODS assay decentralisation process in three health regions in Peru","volume":"15","author":"Mendoza A","year":"2011","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_2_7_9_1","first-page":"482","article-title":"Diagnostic accuracy of the microscopic observation drug susceptibility assay: a pilot study from India","volume":"14","author":"Michael JS","year":"2010","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00687-10"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa055524"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1596\/978-0-8213-8316-2"},{"key":"e_1_2_7_13_1","unstructured":"World Health Organization.Tuberculosis country profiles: China.2011. [Accessed 13 Apr 2011.] Available from URL:https:\/\/extranet.who.int\/sree\/Reports?op=Replet&name=%2FWHO_HQ_Reports%2FG2%2FPROD%2FEXT%2FTBCountryProfile&ISO2=CN&outtype=pdf"},{"key":"e_1_2_7_14_1","volume-title":"Guideline of Clinical Diagnosis and Treatment: Tuberculosis Section","author":"Chinese Medical Association","year":"2005"},{"key":"e_1_2_7_15_1","volume-title":"Public Health Mycobacteriology: A Guide for the Level III Laboratory","author":"Kent PT","year":"1985"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.12.4750-4752.2002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001173"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00829-06"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.4103\/0022-3859.101379"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0404.2009.01316.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2010.01723.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.09.067801"},{"key":"e_1_2_7_23_1","volume-title":"Tuberculosis Laboratory Biosafety Manual","author":"World Health Organization","year":"2012"}],"container-title":["Respirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fresp.12192","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/resp.12192","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T18:18:24Z","timestamp":1696184304000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/resp.12192"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,23]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/resp.12192"],"URL":"https:\/\/doi.org\/10.1111\/resp.12192","archive":["Portico"],"relation":{},"ISSN":["1323-7799","1440-1843"],"issn-type":[{"value":"1323-7799","type":"print"},{"value":"1440-1843","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,23]]}}}