{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:00:59Z","timestamp":1789578059837,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,30]],"date-time":"2015-03-30T00:00:00Z","timestamp":1427673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Recent advances in cultivation\u2010independent molecular biological modalities for detecting bacterial species have indicated that several bacterial species may play a role in the pathogenesis of certain infectious diseases. The aim of this study was to evaluate the role of bacterial flora in the pathogenesis of nontuberculous mycobacteriosis (<jats:styled-content style=\"fixed-case\">NTM<\/jats:styled-content>) using a bacterial floral analysis of bronchoalveolar lavage fluid (<jats:styled-content style=\"fixed-case\">BALF<\/jats:styled-content>) with 16S rRNA gene sequencing in patients with bronchiectasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifty\u2010eight patients with bronchiectasis evaluated using chest computed tomography were enrolled. <jats:styled-content style=\"fixed-case\">BALF<\/jats:styled-content> obtained from the most affected lung lesions was evaluated using culture and culture\u2010independent methodologies. Approximately 600 bp of the bacterial 16S <jats:styled-content style=\"fixed-case\">rRNA<\/jats:styled-content> gene (<jats:styled-content style=\"fixed-case\">E341F\u2010E907R<\/jats:styled-content>) was amplified via polymerase chain reaction using universal primers, and clone libraries were constructed. Nucleotide sequences of 96 randomly chosen clones for each specimen were determined, and the homology was searched using a basic local alignment search tool to determine the bacterial phylotypes and their proportions (bacterial floral analysis) in each specimen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twenty\u2010nine patients with bronchiectasis were diagnosed with <jats:styled-content style=\"fixed-case\">NTM<\/jats:styled-content> based on culture\u2010based methods using Ogawa medium. The molecular method showed a significantly high rate of anaerobes among the patients with <jats:styled-content style=\"fixed-case\">NTM<\/jats:styled-content> compared with that observed in the bronchiectasis patients without <jats:styled-content style=\"fixed-case\">NTM<\/jats:styled-content>. In addition, findings of collapse\/consolidation were significantly related to the proportion of <jats:italic>Prevotella<\/jats:italic> species in the <jats:styled-content style=\"fixed-case\">BALF<\/jats:styled-content> samples determined using the molecular method (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Given the results of the present study, anaerobes may play an important role in the pathogenesis of bronchiectasis in patients with <jats:styled-content style=\"fixed-case\">NTM<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.12536","type":"journal-article","created":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T15:26:25Z","timestamp":1427901985000},"page":"758-765","source":"Crossref","is-referenced-by-count":37,"title":["Possible role of anaerobes in the pathogenesis of nontuberculous mycobacterial infection"],"prefix":"10.1111","volume":"20","author":[{"given":"Kei","family":"Yamasaki","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Mukae","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshinori","family":"Kawanami","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumasa","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Noguchi","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentarou","family":"Akata","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Naito","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keishi","family":"Oda","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takaaki","family":"Ogoshi","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinatsu","family":"Nishida","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Orihashi","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukiko","family":"Kawanami","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ishimoto","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatsumi","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Department of Microbiology University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Yatera","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine University of Occupational and Environmental Health, Japan  Kitakyushu Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200604-571ST"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2008.110957"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063103"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.225.65"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709804104"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05213-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043802"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.10-0460"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032486"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016384"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2009.0100"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201111-2075OC"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2011-201183"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2012-202917"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201104-0655OC"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090934"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00956-13"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-2618-1-27"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.179.3.2027992"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000336238"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.175.11.3247-3252.1993"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.1.79"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2007.07.016"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00330-012-2558-3"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.130.5.1424"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.2214\/ajr.184.4.01841247"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2351040371"}],"container-title":["Respirology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fresp.12536","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/resp.12536","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:14:42Z","timestamp":1695572082000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/resp.12536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,30]]},"references-count":27,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1111\/resp.12536"],"URL":"https:\/\/doi.org\/10.1111\/resp.12536","archive":["Portico"],"relation":{},"ISSN":["1323-7799","1440-1843"],"issn-type":[{"value":"1323-7799","type":"print"},{"value":"1440-1843","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,30]]}}}