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We hypothesized that temsirolimus, an <jats:styled-content style=\"fixed-case\">mTOR<\/jats:styled-content> inhibitor, would curtail experimental mesothelioma progression <jats:italic>in vivo<\/jats:italic> by limiting tumour cell growth, abrogating tumour angiogenesis and modulating immune\/inflammatory tumour milieu.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We produced flank and pleural syngeneic murine mesotheliomas by delivering <jats:styled-content style=\"fixed-case\">AE17<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">AB1<\/jats:styled-content> murine mesothelioma cells into the right flank or the pleural space of <jats:styled-content style=\"fixed-case\">C57BL<\/jats:styled-content>\/6 and <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice, respectively. Animals were given five times\/week intraperitoneal injections of 20\u2009mg\/kg temsirolimus or vehicle and were sacrificed on day 26 (flank) or on day 15 (pleural) post\u2010tumour cell propagation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Temsirolimus limited mesothelioma growth <jats:italic>in vivo<\/jats:italic> by stimulating tumour cell apoptosis, inhibiting tumour angiogenesis, enhancing tumour lymphocyte abundance and blocking pro\u2010tumour myeloid cell recruitment. Pleural fluid accumulation was significantly mitigated in <jats:styled-content style=\"fixed-case\">AE17<\/jats:styled-content> but not in <jats:styled-content style=\"fixed-case\">AB1<\/jats:styled-content> mesotheliomas. <jats:italic>In vitro<\/jats:italic>, temsirolimus hindered mesothelioma cell growth, <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">kappaB<\/jats:styled-content> activation and macrophage migration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In conclusion, temsirolimus apart from inducing tumour cell apoptosis, targets tumour angiogenesis and influences inflammatory tumour microenvironment to halt experimental mesothelioma growth <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.12604","type":"journal-article","created":{"date-parts":[[2015,8,6]],"date-time":"2015-08-06T02:59:45Z","timestamp":1438829985000},"page":"1263-1271","source":"Crossref","is-referenced-by-count":12,"title":["Temsirolimus targets multiple hallmarks of cancer to impede mesothelioma growth <i>in vivo<\/i>"],"prefix":"10.1111","volume":"20","author":[{"given":"Maria Eleni","family":"Vazakidou","sequence":"first","affiliation":[{"name":"\u2018Marianthi Simou Laboratory\u2019 1st Department of Critical Care and Pulmonary Medicine National and Kapodistrian University of Athens School of Medicine Evangelismos Hospital  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophia","family":"Magkouta","sequence":"additional","affiliation":[{"name":"\u2018Marianthi Simou Laboratory\u2019 1st Department of Critical Care and Pulmonary Medicine National and Kapodistrian University of Athens School of Medicine 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