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We aimed to assess the predictive factors of molecular analysis feasibility (MAF<\/jats:styled-content>) from samples of peripheral nonS<\/jats:styled-content>q\u2010NSCLC<\/jats:styled-content> obtained by radial endobronchial ultrasound bronchoscopy (r\u2010EBUS<\/jats:styled-content>) and 1.5\u2009mm microbiopsy forceps.<\/jats:p><\/jats:sec>Methods<\/jats:title>We reviewed data from consecutive peripheral lung nodules sampled with r\u2010EBUS<\/jats:styled-content> between J<\/jats:styled-content>anuary 2012 and J<\/jats:styled-content>uly 2014 at a single F<\/jats:styled-content>rench U<\/jats:styled-content>niversity H<\/jats:styled-content>ospital. nonS<\/jats:styled-content>q\u2010NSCLC<\/jats:styled-content> were systematically analysed for EGFR<\/jats:italic><\/jats:styled-content>,<\/jats:italic>\u2005KRAS<\/jats:italic><\/jats:styled-content>,<\/jats:italic>\u2005ALK<\/jats:italic><\/jats:styled-content>,<\/jats:italic>\u2005HER<\/jats:italic><\/jats:styled-content>2, PI3K<\/jats:italic> and BRAF<\/jats:italic><\/jats:styled-content> throughout the study, and c\u2010<\/jats:italic>MET<\/jats:italic><\/jats:styled-content> and ROS<\/jats:italic><\/jats:styled-content>1<\/jats:italic> alterations for the last 10 months.<\/jats:p><\/jats:sec>Results<\/jats:title>Of 111 nonSq\u2010NSCLC<\/jats:styled-content> diagnosed by r\u2010EBUS<\/jats:styled-content> (113 procedures, mean nodule diameter 28\u2009\u00b1\u200915\u2009mm), 88 were analysed for EGFR<\/jats:italic><\/jats:styled-content> and ALK<\/jats:italic><\/jats:styled-content>, 87 for KRAS<\/jats:italic><\/jats:styled-content>,<\/jats:italic> 86 for HER<\/jats:italic><\/jats:styled-content>2,<\/jats:italic>\u2005PI3K<\/jats:italic><\/jats:styled-content> and BRAF<\/jats:italic><\/jats:styled-content> and 14 for c\u2010MET<\/jats:italic><\/jats:styled-content>. Forty\u2010one mutations were identified (23 KRAS<\/jats:italic><\/jats:styled-content>, 10 EGFR<\/jats:italic><\/jats:styled-content>, 2 BRAF<\/jats:italic><\/jats:styled-content>, 1 HER<\/jats:italic><\/jats:styled-content>2<\/jats:italic> and 5 ALK<\/jats:italic><\/jats:styled-content> rearrangements). Four c\u2010<\/jats:italic>MET<\/jats:italic><\/jats:styled-content> overexpressions were noted. MAF<\/jats:styled-content> rose from 67% for the first 57 procedures to 89% for the last 56 procedures (P<\/jats:italic>\u2009=\u20090.02) likely due to a higher number of biopsies performed (2\u2009\u00b1\u20091 vs 3\u2009\u00b1\u20092, P<\/jats:italic>\u2009=\u20090.005). Upper or middle lobe location (OR<\/jats:styled-content> 1.19, 95% CI: 1.02\u20131.38, P<\/jats:italic>\u2009=\u20090.03), and at least three biopsies (OR<\/jats:styled-content> 1.20, 95% CI: 1.04\u20131.40, P<\/jats:italic>\u2009=\u20090.02) were predictive factors of MAF<\/jats:styled-content>. Percentage of tumour cells, size of lesion and distance to the pleura did not correlate with MAF<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Multi\u2010gene molecular analysis could be performed in nearly 80% of paraffin\u2010embedded biopsies or smear specimens sampled by r\u2010EBUS<\/jats:styled-content> assisted bronchoscopy of peripheral tumoral lung nodules.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.12737","type":"journal-article","created":{"date-parts":[[2016,1,27]],"date-time":"2016-01-27T09:11:29Z","timestamp":1453885889000},"page":"718-726","source":"Crossref","is-referenced-by-count":33,"title":["Molecular analysis of peripheral non\u2010squamous non\u2010small cell lung cancer sampled by radial EBUS<\/scp>"],"prefix":"10.1111","volume":"21","author":[{"given":"Florian","family":"Guisier","sequence":"first","affiliation":[{"name":"Clinique Pneumologique Rouen University Hospital Rouen France"},{"name":"CIC\u2010INSERM 1404 Rouen University Hospital Rouen France"},{"name":"EA 4108, LITIS QuantiF laboratory University of Rouen Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Sala\u00fcn","sequence":"additional","affiliation":[{"name":"Clinique Pneumologique Rouen University Hospital Rouen France"},{"name":"CIC\u2010INSERM 1404 Rouen University Hospital Rouen France"},{"name":"EA 4108, LITIS QuantiF laboratory University of Rouen Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samy","family":"Lachkar","sequence":"additional","affiliation":[{"name":"Clinique Pneumologique Rouen University Hospital Rouen France"},{"name":"CIC\u2010INSERM 1404 Rouen University Hospital Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aude","family":"Lamy","sequence":"additional","affiliation":[{"name":"Genetic Somatic Tumor Laboratory and Pathology Department Rouen University Hospital Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Piton","sequence":"additional","affiliation":[{"name":"Genetic Somatic Tumor Laboratory and Pathology Department Rouen University Hospital Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9reng\u00e8re","family":"Obstoy","sequence":"additional","affiliation":[{"name":"Clinique Pneumologique Rouen University Hospital Rouen France"},{"name":"CIC\u2010INSERM 1404 Rouen University Hospital Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Christophe","family":"Sabourin","sequence":"additional","affiliation":[{"name":"Genetic Somatic Tumor Laboratory and Pathology Department Rouen University Hospital Rouen France"},{"name":"INSERM U1079, IRIB Rouen University Hospital Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Thiberville","sequence":"additional","affiliation":[{"name":"Clinique Pneumologique Rouen University Hospital Rouen France"},{"name":"CIC\u2010INSERM 1404 Rouen University Hospital Rouen France"},{"name":"EA 4108, LITIS QuantiF laboratory University of Rouen Rouen France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdu145"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.57.3055"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e318290868f"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdu123"},{"key":"e_1_2_7_6_1","unstructured":"National Comprehensive Cancer Network (NCCN).NCCN Clinical Practice Guidelines in Oncology. 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