{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T19:42:51Z","timestamp":1780602171519,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,11,22]],"date-time":"2016-11-22T00:00:00Z","timestamp":1479772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Alan King Westcare Project Grant"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"\nABSTRACT<\/jats:styled-content>\n<\/jats:title>Background and objective<\/jats:title>Exudative pleural effusions affect over 1500 patients per million population each year. The pathobiology of pleural exudate formation remains unclear. Our recent study revealed monocyte chemotactic protein\u20101 (MCP<\/jats:styled-content>\u20101) as a key driver of fibrinolytic\u2010induced exudate effusion while another study found a role for MCP<\/jats:styled-content>\u20101 in malignant effusion formation. In the present study, we further evaluated the role of MCP<\/jats:styled-content>\u20101 in the development of pleural effusion in a mouse model of acute pleural inflammation.<\/jats:p><\/jats:sec>Methods<\/jats:title>\u03bb\u2010Carrageenan (CAR<\/jats:styled-content>) was injected into the pleural cavity of CD1<\/jats:styled-content> mice and pleural effusion volume measured up to 16\u2009h post\u2010injection. Pleural effusion and serum protein and MCP<\/jats:styled-content>\u20101 concentrations were measured and differential cell counts performed in fluids. Mice were also treated with either intraperitoneal (i) anti\u2010MCP<\/jats:styled-content>\u20101 antibody or isotype control or (ii) an MCP<\/jats:styled-content>\u20101 receptor (CCR2<\/jats:styled-content>) antagonist or vehicle control 12\u2009h prior to and at the time of CAR<\/jats:styled-content> injection.<\/jats:p><\/jats:sec>Results<\/jats:title>Intrapleural CAR<\/jats:styled-content> induced significant pleural fluid accumulation (300.0\u2009\u00b1\u200949.9\u2009\u03bcL<\/jats:styled-content>) in mice after 4\u2009h. Pleural fluid MCP<\/jats:styled-content>\u20101 concentrations were significantly higher than corresponding serum MCP<\/jats:styled-content>\u20101 (144\u2009603\u2009\u00b1 23\u2009204\u2009pg\/mL<\/jats:styled-content> vs 3703\u2009\u00b1\u2009801\u2009pg\/mL<\/jats:styled-content>, P<\/jats:italic>\u2009<\u20090.0001). A significant decrease in pleural fluid formation was seen both with anti\u2010MCP<\/jats:styled-content>\u20101 antibody (median (interquartile range, IQR<\/jats:styled-content>): 36 (0\u2013168) \u03bcL<\/jats:styled-content> vs controls 290 (70\u2013436) \u03bcL<\/jats:styled-content>; P<\/jats:italic>\u2009=\u20090.02) or CCR2<\/jats:styled-content> antagonist (153 (30\u2013222) \u03bcL<\/jats:styled-content> vs controls 240 (151\u2013331) \u03bcL<\/jats:styled-content>, P<\/jats:italic>\u2009=\u20090.0049).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Blockade of MCP<\/jats:styled-content>\u20101 activity significantly reduced inflammatory pleural effusion formation in a CAR<\/jats:styled-content> model. Together with recent successes in MCP<\/jats:styled-content>\u20101 blockade in other effusion formation models, our data strongly support clinical evaluation of MCP<\/jats:styled-content>\u20101 antagonists as a novel approach to pleural fluid management.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.12951","type":"journal-article","created":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T01:59:48Z","timestamp":1479866388000},"page":"758-763","source":"Crossref","is-referenced-by-count":18,"title":["Role of MCP<\/scp>\u20101 in pleural effusion development in a carrageenan\u2010induced murine model of pleurisy"],"prefix":"10.1111","volume":"22","author":[{"given":"Sally M.","family":"Lansley","sequence":"first","affiliation":[{"name":"Centre for Respiratory Health, School of Medicine & Pharmacology University of Western Australia Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui Min","family":"Cheah","sequence":"additional","affiliation":[{"name":"Centre for Respiratory Health, School of Medicine & Pharmacology University of Western Australia Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.C. Gary","family":"Lee","sequence":"additional","affiliation":[{"name":"Centre for Respiratory Health, School of Medicine & Pharmacology University of Western Australia Perth Western Australia Australia"},{"name":"Respiratory Department Sir Charles Gairdner Hospital Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","DOI":"10.1201\/b19146","volume-title":"Textbook of Pleural Diseases","author":"Light R","year":"2016"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thorsurg.2012.10.004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2008.0027"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2014-0017OC"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djn325"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200804-616ED"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.7.1383"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4161\/onci.29195"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00658.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.141.4.7404"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2004.05.008"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2011.02.004"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"5094","DOI":"10.4049\/jimmunol.163.9.5094","article-title":"Role of IL\u20106 in the pleurisy and lung injury caused by carrageenan","volume":"163","author":"Cuzzocrea S","year":"1999","journal-title":"J. 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