{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:43:14Z","timestamp":1792629794538,"version":"4.5.0"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,3,22]],"date-time":"2017-03-22T00:00:00Z","timestamp":1490140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"\nABSTRACT<\/jats:styled-content>\n<\/jats:title>Background and objective<\/jats:title>The objective of this study was to enumerate total cells and the number of inflammatory cell differentials in large airways (LAs<\/jats:styled-content>) versus small airways (SAs<\/jats:styled-content>) of mild\u2010moderate COPD<\/jats:styled-content>, and against appropriate controls.<\/jats:p><\/jats:sec>Methods<\/jats:title>For LA<\/jats:styled-content>, we used endobronchial biopsies and for SA<\/jats:styled-content> resected lung tissues. Immunostaining was enumerated (cells per mm2<\/jats:sup>) for macrophages, neutrophils, CD4<\/jats:styled-content> and CD8<\/jats:styled-content> T cells in the lamina propria (LP<\/jats:styled-content>) up to 150\u2009\u00b5M deep for LA<\/jats:styled-content> and full wall thickness for SA<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>We confirmed hypocellularity in the LA<\/jats:styled-content> and in the SA<\/jats:styled-content> wall in smokers and COPD<\/jats:styled-content> (P<\/jats:italic>\u2009<\u20090.001). LA<\/jats:styled-content> cellularity was least in current smokers with COPD<\/jats:styled-content> (COPD\u2010CS<\/jats:styled-content>) (P<\/jats:italic>\u2009<\u20090.01), while SA<\/jats:styled-content> cellularity was similar across smoker\/COPD<\/jats:styled-content> groups. LA<\/jats:styled-content> neutrophils were decreased in COPD\u2010CS<\/jats:styled-content> (P<\/jats:italic>\u2009<\u20090.01), while SA<\/jats:styled-content> neutrophil counts were unchanged. Compared with controls, LA<\/jats:styled-content> macrophage numbers in COPD<\/jats:styled-content> were significantly lower (P<\/jats:italic>\u2009<\u20090.05), with SA<\/jats:styled-content> macrophage numbers unchanged. A significant increase was observed in SA CD8<\/jats:styled-content>+ cells in both normal smokers (P<\/jats:italic>\u2009<\u20090.01) and COPD\u2010CS<\/jats:styled-content> (P<\/jats:italic>\u2009<\u20090.001) but not in LA<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>These unique data indicate that the current model for airway wall inflammation in COPD<\/jats:styled-content> is oversimplified, and contrast with innate inflammatory activation in the lumen, at least in mild\u2010moderate disease. Any abnormalities in airway wall cell differentials are small, although exaggerated in percentage terms.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.13021","type":"journal-article","created":{"date-parts":[[2017,3,22]],"date-time":"2017-03-22T21:59:06Z","timestamp":1490219946000},"page":"1125-1132","source":"Crossref","is-referenced-by-count":53,"title":["Profiling cellular and inflammatory changes in the airway wall of mild to moderate COPD<\/scp>"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0570-7059","authenticated-orcid":false,"given":"Mathew S.","family":"Eapen","sequence":"first","affiliation":[{"name":"NHMRC Centre of Research Excellence for Chronic Respiratory Disease, School of Medicine University of Tasmania Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kielan","family":"McAlinden","sequence":"additional","affiliation":[{"name":"NHMRC Centre of Research Excellence for Chronic Respiratory Disease, School of Medicine University of Tasmania Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Tan","sequence":"additional","affiliation":[{"name":"NHMRC Centre of Research Excellence for Chronic Respiratory Disease, School of Medicine University of Tasmania Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Weston","sequence":"additional","affiliation":[{"name":"NHMRC Centre of Research Excellence for Chronic Respiratory Disease, School of Medicine University of Tasmania Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Ward","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Newcastle University Newcastle upon Tyne UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans K.","family":"Muller","sequence":"additional","affiliation":[{"name":"NHMRC Centre of Research Excellence for Chronic Respiratory Disease, School of Medicine University of Tasmania Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene H.","family":"Walters","sequence":"additional","affiliation":[{"name":"NHMRC Centre of Research Excellence for Chronic Respiratory Disease, School of Medicine University of Tasmania Hobart Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukhwinder S.","family":"Sohal","sequence":"additional","affiliation":[{"name":"NHMRC Centre of Research Excellence for Chronic Respiratory Disease, School of Medicine University of Tasmania Hobart Tasmania Australia"},{"name":"School of Health Sciences, Faculty of Health University of Tasmania Launceston Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1586\/ers.13.26"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/COPD.S81032"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.98.12020380"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.2000.15a20.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0954-6111(98)90389-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2009.133363"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.158.4.9802078"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2001.01098.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.160.2.9812020"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064833"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.155.3.9117016"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.158.5.9802121"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/15412555.2013.836166"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2003.013029"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/resp.12434"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2004.024463"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200509-1525OC"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032158"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2010.157065"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1309\/QHD6YFN81KQXUUH6"},{"key":"e_1_2_8_22_1","first-page":"215","article-title":"The \"classical\" macrophage marker CD68 is strongly expressed in primary human fibroblasts","volume":"87","author":"Kunz\u2010Schughart LA","year":"2003","journal-title":"Verh. 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