{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:45:27Z","timestamp":1791981927114,"version":"4.4.0"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T00:00:00Z","timestamp":1491350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["T32\u2010HL066988\u20101"],"award-info":[{"award-number":["T32\u2010HL066988\u20101"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Pulmonary infiltrates are common in immunosuppressed patients. Bronchoscopy with bronchoalveolar lavage (<jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content>) is often used to evaluate their aetiology. However, it may not always be easily performed. Thus, alternative diagnostic strategies may be needed. There is limited data on the correlation of nasopharyngeal (<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>) respiratory viral panel (<jats:styled-content style=\"fixed-case\">RVP<\/jats:styled-content>)\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> testing compared with <jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content>. We aimed to identify the predictive value of <jats:styled-content style=\"fixed-case\">NP RVP\u2010PCR<\/jats:styled-content> samples compared with samples obtained from <jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content> in immunosuppressed patients with pulmonary infiltrates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We conducted an observational retrospective study of immunosuppressed adults who underwent bronchoscopy in the Pulmonary Department at the University of Rochester Medical Center between January 2011 and June 2016. We compared the positive and negative predictive values, sensitivity, specificity and false negative rate of <jats:styled-content style=\"fixed-case\">NP RVP\u2010PCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">BAL RVP\u2010PCR<\/jats:styled-content>, as well as identified clinical predictors of positive viral <jats:styled-content style=\"fixed-case\">BAL RVP\u2010PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eighty\u2010nine immunosuppressed patients had both <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> and bronchoalveolar <jats:styled-content style=\"fixed-case\">RVP\u2010PCR<\/jats:styled-content> testing. Twenty\u2010one patients had <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>(+)<jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content>(+) <jats:styled-content style=\"fixed-case\">RVP\u2010PCR<\/jats:styled-content> testing. Seven patients had false negative (<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>(\u2212)<jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content>(+)) <jats:styled-content style=\"fixed-case\">RVP\u2010PCR<\/jats:styled-content> testing. Three patients had <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>(+)<jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content>(\u2212) <jats:styled-content style=\"fixed-case\">RVP\u2010PCR<\/jats:styled-content> testing. The positive and negative predictive values of <jats:styled-content style=\"fixed-case\">NP RVP\u2010PCR<\/jats:styled-content> testing were 88% and 89%, respectively. Allogeneic bone marrow transplantation and testing performed in the winter and spring months were significantly associated with positive <jats:styled-content style=\"fixed-case\">BAL RVP\u2010PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>\u2009=\u20093.3 (1.19\u20139.12); <jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>\u2009=\u20094.62 (1.64\u201312.99), respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>\n<jats:styled-content style=\"fixed-case\">NP RVP\u2010PCR<\/jats:styled-content> testing has high concordance with testing performed on <jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content> samples. Repeat testing through <jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content> is beneficial when there is high concern for viral infection after initial <jats:styled-content style=\"fixed-case\">NP RVP\u2010PCR<\/jats:styled-content> testing is negative.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.13049","type":"journal-article","created":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T00:52:41Z","timestamp":1491439961000},"page":"1205-1211","source":"Crossref","is-referenced-by-count":31,"title":["Nasopharyngeal viral <scp>PCR<\/scp> in immunosuppressed patients and its association with virus detection in bronchoalveolar lavage by <scp>PCR<\/scp>"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3441-8264","authenticated-orcid":false,"given":"Daniel J.","family":"Lachant","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine University of Rochester Medical Center\/Strong Memorial Hospital  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel P.","family":"Croft","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine University of Rochester Medical Center\/Strong Memorial Hospital  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"McGrane Minton","sequence":"additional","affiliation":[{"name":"Department of Public Health Sciences University of Rochester  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paritosh","family":"Prasad","sequence":"additional","affiliation":[{"name":"Department of Transplant Infectious Disease University of Rochester Medical Center\/Strong Memorial Hospital  Rochester New York USA"},{"name":"Department of Critical Care Medicine University of Rochester Medical Center\/Strong Memorial Hospital  Rochester New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Kottmann","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine University of Rochester Medical Center\/Strong Memorial Hospital  Rochester New York 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