{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T07:09:34Z","timestamp":1760080174685,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,4,24]],"date-time":"2018-04-24T00:00:00Z","timestamp":1524528000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Department of Science and Technology of Liaoning Province in China","award":["2014225006"],"award-info":[{"award-number":["2014225006"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Delayed diagnosis and treatment of tuberculosis (TB) contribute to poor outcomes, especially for endobronchial TB (EBTB), which typically leads to tracheobronchial stenosis. Finding rapid and accurate diagnostic tools for EBTB is crucial. GeneXpert <jats:italic>Mycobacterium tuberculosis<\/jats:italic> (MTB)\/rifampin (RIF) was recommended by the World Health Organization (WHO) as a standard molecular biological diagnostic technique for MTB. The aim of this study was to evaluate the efficacy of GeneXpert MTB\/RIF for diagnosing EBTB and for evaluating RIF resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Biopsy tissue and bronchial brushings from EBTB patients were prospectively assessed with GeneXpert MTB\/RIF. The diagnostic yields of auramine O\u2010stained sputum smears and bronchial brush smears were obtained, and the results were compared with the cultures of sputum and biopsy tissues for MTB.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In 61 confirmed cases of EBTB, the sensitivities of sputum smear, bronchial brush smear, sputum culture and tissue culture to diagnose EBTB were 13.1%, 32.8%, 36.1% and 68.9%, respectively. For bronchial brushings and biopsies, our data showed sensitivities of 57.4% and 63.9%, respectively, and a specificity of 100% for GeneXpert MTB\/RIF, and these results were superior to those of sputum smears, bronchial brush smears and sputum culture. GeneXpert MTB\/RIF for bronchial brushings and biopsies showed complementarity in its diagnostic performance. Resistance to RIF was identified in 17.4% (8\/46) of GeneXpert MTB\u2010positive cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>GeneXpert MTB\/RIF may enable more rapid EBTB diagnosis and determination of RIF resistance, which are crucial for timely treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.13316","type":"journal-article","created":{"date-parts":[[2018,4,25]],"date-time":"2018-04-25T02:21:25Z","timestamp":1524622885000},"page":"950-955","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["GeneXpert MTB\/RIF for rapid diagnosis and rifampin resistance detection of endobronchial tuberculosis"],"prefix":"10.1111","volume":"23","author":[{"given":"Qin","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Respiratory Diseases The First Hospital of China Medical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Endoscopy Shenyang Thoracic Hospital  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing\u2010qi","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Laboratory Shenyang Thoracic Hospital  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Endoscopy Shenyang Thoracic Hospital  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"An\u2010na","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Endoscopy Shenyang Thoracic Hospital  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010han","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Endoscopy Shenyang Thoracic Hospital  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Endoscopy Shenyang Thoracic Hospital  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Shenyang Thoracic Hospital  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Kang","sequence":"additional","affiliation":[{"name":"Institute of Respiratory Diseases The First Hospital of China Medical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3438-1764","authenticated-orcid":false,"given":"Gang","family":"Hou","sequence":"additional","affiliation":[{"name":"Institute of Respiratory Diseases The First Hospital of China Medical University  Shenyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,4,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00131406"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1205-2"},{"key":"e_1_2_7_4_1","first-page":"764","article-title":"Characteristics of endobronchial tuberculosis patients with negative sputum acid\u2010fast bacillus","volume":"5","author":"Sahin F","year":"2013","journal-title":"J. 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