{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T16:14:11Z","timestamp":1793031251801,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T00:00:00Z","timestamp":1549929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1 U54 GM104940"],"award-info":[{"award-number":["1 U54 GM104940"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1P306M106392\u201001A1"],"award-info":[{"award-number":["1P306M106392\u201001A1"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Endothelial microparticles (EMP) are submicron vesicles released from endothelial cells. We aimed to determine the utility of EMP as biomarkers of pulmonary arterial hypertension (PAH) in systemic sclerosis (SSc) patients and the pathogenic role of microparticles (MP) in vascular inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Levels of EMP (CD144+, CD31+, CD62E+ and CD143+) were compared between three groups (10 SSc patients with PAH, 10 SSc patients without pulmonary hypertension (no\u2010PH) and 10 healthy age\u2010 and sex\u2010matched controls). Human pulmonary artery endothelial cells (HPAEC) were exposed in vitro to MP obtained from SSc patients or healthy controls, and levels of cytokines and inflammatory adhesion molecules were compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD144+ EMP were significantly higher in the SSc\u2010PAH group compared to either the SSc\u2010no PH or healthy controls (diagnostic accuracy 80%, <jats:italic>P<\/jats:italic>\u2009=\u20090.02). Compared to controls, SSc patients had higher CD31+\/CD62E+ ratios, indicating larger contributions of apoptosis to EMP release (<jats:italic>P<\/jats:italic>\u2009=\u20090.04). Patients with limited SSc had significantly higher levels of CD143+ EMP compared to those with diffuse subtype (<jats:italic>P<\/jats:italic>\u2009=\u20090.008). When HPAEC were exposed to MP from SSc patients, there was a significant increase in inflammatory cytokines and adhesion molecules. Interestingly, exposure to healthy control MP caused a reduction in inflammatory markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>EMP (particularly CD144+) are promising biomarkers of PAH in SSc but require further study. MP isolated from SSc patients induced an increase in endothelial cell inflammation and may be an important pathogenic factor in SSc.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.13500","type":"journal-article","created":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T11:55:08Z","timestamp":1549972508000},"page":"675-683","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Microparticles in systemic sclerosis: Potential pro\u2010inflammatory mediators and pulmonary hypertension biomarkers"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5125-1801","authenticated-orcid":false,"given":"Matthew R.","family":"Lammi","sequence":"first","affiliation":[{"name":"Section of Pulmonary\/Critical Care and Allergy Immunology Louisiana State University Health Sciences Center  New Orleans LA USA"},{"name":"Comprehensive Pulmonary Hypertension Center\u2010University Medical Center  New Orleans LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesley Ann","family":"Saketkoo","sequence":"additional","affiliation":[{"name":"Comprehensive Pulmonary Hypertension Center\u2010University Medical Center  New Orleans LA USA"},{"name":"Tulane University School of Medicine New Orleans Scleroderma and Sarcoidosis Patient Care and Research Center  New Orleans LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel C.","family":"Okpechi","sequence":"additional","affiliation":[{"name":"Stanley S. Scott Cancer Center Louisiana State University Health Sciences Center  New Orleans LA USA"},{"name":"Biochemistry and Molecular Biology School of Graduate Studies, Louisiana State University Health Sciences Center  New Orleans LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Ghonim","sequence":"additional","affiliation":[{"name":"Stanley S. Scott Cancer Center Louisiana State University Health Sciences Center  New Orleans LA USA"},{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy Al\u2010Azhar University  Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Wyczechowska","sequence":"additional","affiliation":[{"name":"Stanley S. 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