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We have previously shown that E\u2010cigarettes cause decreased phagocytosis of bacteria by macrophages via reductions in surface bacterial recognition receptors. This study assessed the effect of E\u2010cigarette constituents, 3 E\u2010liquid apple flavours, nicotine, vegetable glycerine and propylene glycol, on bronchial epithelial cell viability, apoptosis and cytokine secretion and macrophage phagocytosis of apoptotic airway cells and phagocytic recognition molecules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cell necrosis and apoptosis were measured by Sytox Green stain and Annexin V. Efferocytosis was measured by internalization of pHrodo Green labelled apoptotic airway cells by macrophages. Expression of macrophage cell surface apoptotic cell receptors was measured by flow cytometry. Cytokine release by E\u2010cigarette\u2010exposed airway cells was measured by cytokine bead array.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>E\u2010cigarette vapour increased primary bronchial epithelial necrosis and apoptosis. E\u2010cigarette vapour reduced efferocytosis (lowest flavour 12.1%) versus control (20.2%, <jats:italic>P<\/jats:italic> = 0.032). The efferocytosis receptor CD44 was reduced by one flavour (MFI 1863 vs 2332 control, <jats:italic>P<\/jats:italic> = 0.016) and all components reduced expression of CD36, including the glycol bases (MFI 1067\u201312\u2009274 vs 1415 control). Reduced secretion of TNF\u2010\u03b1, IL\u20106, IP\u201010, MIP\u20101\u03b1 and MIP\u20101\u03b2 was observed for all flavour variants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>E\u2010cigarettes can cause bronchial epithelial apoptosis and macrophage efferocytosis dysfunction via reduced expression of apoptotic cell recognition receptors. These data further show that E\u2010cigarettes should not be considered harmless to non\u2010smokers and their effects may go far beyond cytotoxicity to cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.13696","type":"journal-article","created":{"date-parts":[[2019,9,22]],"date-time":"2019-09-22T23:00:52Z","timestamp":1569193252000},"page":"620-628","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Effects of E\u2010cigarette E\u2010liquid components on bronchial epithelial cells: Demonstration of dysfunctional efferocytosis"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0600-4585","authenticated-orcid":false,"given":"Miranda P.","family":"Ween","sequence":"first","affiliation":[{"name":"Department of Thoracic Medicine Royal Adelaide Hospital  Adelaide SA Australia"},{"name":"School of Medicine University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhys","family":"Hamon","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine Royal Adelaide Hospital  Adelaide SA Australia"},{"name":"School of Medicine University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew G.","family":"Macowan","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine Royal Adelaide Hospital  Adelaide SA Australia"},{"name":"School of Medicine University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leigh","family":"Thredgold","sequence":"additional","affiliation":[{"name":"Department of Occupational and Environmental Health, School of Public Health University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2273-1774","authenticated-orcid":false,"given":"Paul N.","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Department of Thoracic Medicine Royal Adelaide Hospital  Adelaide SA Australia"},{"name":"School of Medicine University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra J.","family":"Hodge","sequence":"additional","affiliation":[{"name":"School of Medicine University of Adelaide  Adelaide SA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.13370"},{"key":"e_1_2_9_3_1","unstructured":"WalsbergerS HavillM. 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