{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T05:20:08Z","timestamp":1778563208547,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T00:00:00Z","timestamp":1583107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background and objective<\/jats:title><jats:p>Mesothelial cells and cardiomyocytes have shared embryonic mesodermal origin. Cardiomyocytes release BNP under stretch. We searched whether malignant mesothelioma cells also secrete BNP and if so, this has a meaningful impact.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Part I: Prospectively, patients with pleural lesions on CT having malignant mesothelioma effusions (MME, <jats:italic>n<\/jats:italic> = 13) were compared to patients with malignant effusions with pleural lesions (MEa, <jats:italic>n<\/jats:italic> = 14). Age\u2010matched patients with ME without pleural lesions (MEb, <jats:italic>n<\/jats:italic> = 16) and non\u2010malignant effusions (NME, <jats:italic>n<\/jats:italic> = 25) were analysed. Part II: Retrospectively, samples from patients with mesothelioma (<jats:italic>n<\/jats:italic> = 14), lung cancer (<jats:italic>n<\/jats:italic> = 8) or heart failure (<jats:italic>n<\/jats:italic> = 9) were used. BNP was measured in pleural fluid and blood\/plasma. Part III: BNP was assessed in the culture supernatants of benign (MeT\u20105A) and malignant mesothelioma cell lines (M14K\u2010epithelioid, MSTO\u2010biphasic and ZL34\u2010sarcomatoid) (<jats:italic>n<\/jats:italic> = 10 per cell line in three different biological replicates).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In vitro, BNP concentration was significantly higher in the supernatant of all malignant cell lines than benign ones (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), denoting BNP's production from the former. The pleural fluid to blood BNP ratio in MME was extremely high in Part I and Part II subjects (28.3\u2009\u00b1\u200912.1 and 25.9\u2009\u00b1\u20098.6, respectively) versus 1.1\u2009\u00b1\u20090.3 and 0.4\u2009\u00b1\u20090.1 in Part I ME and NME, respectively (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001), and 0.8\u2009\u00b1\u20090.1 and 0.4\u2009\u00b1\u20090.1 in Part II ME and NME, respectively (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001). BNP ratio \u22652.11 in Part I had 92% sensitivity and 94.5% specificity for MME (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>BNP is secreted from malignant mesothelial cells. In clinical practice, the pleural fluid to blood BNP ratio can help in the diagnosis of malignant mesothelioma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.13788","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T03:59:39Z","timestamp":1583207979000},"page":"1060-1065","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Malignant mesothelioma cells secrete natriuretic peptides: Data and diagnostic clinical implications"],"prefix":"10.1111","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2412-5388","authenticated-orcid":false,"given":"Vasiliki","family":"Tsolaki","sequence":"first","affiliation":[{"name":"Intensive Care Unit, University Hospital of Larissa, Faculty of Medicine University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sotirios","family":"Zarogiannis","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paris","family":"Zygoulis","sequence":"additional","affiliation":[{"name":"Intensive Care Unit, University Hospital of Larissa, Faculty of Medicine University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioannis","family":"Kalomenidis","sequence":"additional","affiliation":[{"name":"1<sup>st<\/sup> Department of Critical Care and Pulmonary Medicine \u2018Evangelismos Hospital\u2019, National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajesh","family":"Jagirdar","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Demosthenes","family":"Makris","sequence":"additional","affiliation":[{"name":"Intensive Care Unit, University Hospital of Larissa, Faculty of Medicine University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoe","family":"Daniil","sequence":"additional","affiliation":[{"name":"Respiratory Medicine Department, University Hospital of Larissa, Faculty of Medicine University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7143-3311","authenticated-orcid":false,"given":"Sofia","family":"Magkouta","sequence":"additional","affiliation":[{"name":"1<sup>st<\/sup> Department of Critical Care and Pulmonary Medicine \u2018Evangelismos Hospital\u2019, National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ioannis","family":"Triantafyllou","sequence":"additional","affiliation":[{"name":"Department of Computer Science and Biomedical Informatics, School of Sciences University of Thessaly  Lamia Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Papanikolaou","sequence":"additional","affiliation":[{"name":"Intensive Care Unit, University Hospital of Larissa, Faculty of Medicine University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Konstantinos I.","family":"Gourgoulianis","sequence":"additional","affiliation":[{"name":"Respiratory Medicine Department, University Hospital of Larissa, Faculty of Medicine University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Epaminondas","family":"Zakynthinos","sequence":"additional","affiliation":[{"name":"Intensive Care Unit, University Hospital of Larissa, Faculty of Medicine University of Thessaly  Larissa Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.06.00113205"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-0270"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2003.11.008"},{"key":"e_1_2_8_5_1","unstructured":"PonikowskiP VoorsAA AnkerSD BuenoH ClelandJGF CoatsAJS FalkV Gonzalez\u2010JuanateyJR HarjolaVP JankowskaEAet al.;ESC Scientififc Document Group. 2016 ESC guidelines for the diagnosis and treatment of acute and chronic heart failure: the Task Force for the diagnosis and treatment of acute and chronic heart failure of the European Society of Cardiology (ESC). 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