{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T16:21:19Z","timestamp":1793722879424,"version":"4.6.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T00:00:00Z","timestamp":1708387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Respirology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objective<\/jats:title><jats:p>Next\u2010generation sequencing (NGS) analysis is considered standard for lung cancer diagnosis in clinical practice. Little is known about the feasibility of NGS using tumour tissue sampled with a 1.1\u2009mm\u2010diameter cryoprobe. We aimed to investigate the suitability of specimens obtained by transbronchial cryobiopsy (TBC) using a 1.1\u2009mm\u2010diameter cryoprobe for NGS analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with lung cancer who underwent TBC using a 1.1\u2009mm\u2010diameter cryoprobe for NGS testing between October 2020 and April 2023 were enrolled. A 4.0\u2010 or 3.0\u2009mm\u2010diameter bronchoscope with radial probe endobronchial ultrasound and virtual bronchoscopic navigation was used to detect peripheral lung lesions. All procedures were performed under fluoroscopic guidance. Data were analysed retrospectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 56 patients underwent TBC using a 1.1\u2009mm cryoprobe for NGS testing, during the study period. Most patients (98%) were in the advanced stage of lung cancer (recurrent or inoperable disease of stages III or IV). The diagnostic yield of NGS for DNA and RNA sequencing was 95% each (53 of 56). Moderate bleeding was noted in three patients (5%) and none of the study patients developed life\u2010threatening complications, such as pneumothorax or lung infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TBC using a 1.1\u2009mm\u2010diameter cryoprobe is a useful and safe tool for NGS analysis, for both DNA and RNA sequencing.<\/jats:p><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/resp14680-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/resp.14680","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T05:12:50Z","timestamp":1708492370000},"page":"333-339","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Next\u2010generation sequencing using tissue specimen collected with a 1.1\u2009mm\u2010diameter cryoprobe in patients with lung cancer"],"prefix":"10.1111","volume":"29","author":[{"given":"Mi\u2010Hyun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Pusan National University School of Medicine  Busan Republic of Korea"},{"name":"Department of Internal Medicine Pusan National University Hospital  Busan Republic of Korea"},{"name":"Biomedical Research Institute, Pusan National University Hospital  Busan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Han","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Pusan National University School of Medicine  Busan Republic of Korea"},{"name":"Department of Internal Medicine Pusan National University Hospital  Busan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geewon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Radiology Pusan National University School of Medicine  Busan Republic of Korea"},{"name":"Department of Radiology Pusan National University Hospital  Busan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeongha","family":"Mok","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Pusan National University School of Medicine  Busan Republic of Korea"},{"name":"Department of Internal Medicine Pusan National University Hospital  Busan 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