{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T04:07:14Z","timestamp":1788667634082,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T00:00:00Z","timestamp":1465516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["Ricerca Corrente 2010 funds"],"award-info":[{"award-number":["Ricerca Corrente 2010 funds"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Risk Analysis"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p>Semisoft cheese made from raw sheep's milk is traditionally and economically important in southern Europe. However, raw milk cheese is also a known vehicle of human listeriosis and contamination of sheep cheese with <jats:italic>Listeria monocytogenes<\/jats:italic> has been reported. In the present study, we have developed and applied a quantitative risk assessment model, based on available evidence and challenge testing, to estimate risk of invasive listeriosis due to consumption of an artisanal sheep cheese made with raw milk collected from a single flock in central Italy. In the model, contamination of milk may originate from the farm environment or from mastitic animals, with potential growth of the pathogen in bulk milk and during cheese ripening. Based on the 48\u2010day challenge test of a local semisoft raw sheep's milk cheese we found limited growth only during the initial phase of ripening (24 hours) and no growth or limited decline during the following ripening period. In our simulation, in the baseline scenario, 2.2% of cheese servings are estimated to have at least 1 colony forming unit (CFU) per gram. Of these, 15.1% would be above the current E.U. limit of 100 CFU\/g (5.2% would exceed 1,000 CFU\/g). Risk of invasive listeriosis per random serving is estimated in the 10<jats:sup>\u221212<\/jats:sup> range (mean) for healthy adults, and in the 10<jats:sup>\u221210<\/jats:sup> range (mean) for vulnerable populations. When small flocks (10\u201336 animals) are combined with the presence of a sheep with undetected subclinical mastitis, risk of listeriosis increases and such flocks may represent a public health risk.<\/jats:p>","DOI":"10.1111\/risa.12649","type":"journal-article","created":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T16:57:54Z","timestamp":1465577874000},"page":"661-676","source":"Crossref","is-referenced-by-count":15,"title":["Risk Assessment of Human Listeriosis from Semisoft Cheeses Made from Raw Sheep's Milk in Lazio and Tuscany (Italy)"],"prefix":"10.1111","volume":"37","author":[{"given":"Roberto","family":"Condoleo","sequence":"first","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziad","family":"Mezher","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selene","family":"Marozzi","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Guzzon","sequence":"additional","affiliation":[{"name":"Hylobates Consulting srl  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Fischetti","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matteo","family":"Senese","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Sette","sequence":"additional","affiliation":[{"name":"Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria Centro di Ricerca per gli Alimenti e la Nutrizione  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Bucchini","sequence":"additional","affiliation":[{"name":"Hylobates Consulting srl  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,10]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"EUROSTAT"},{"key":"e_1_2_8_3_1","unstructured":"EUROSTAT"},{"key":"e_1_2_8_4_1","unstructured":"European Commission. 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