{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T18:42:50Z","timestamp":1759776170878,"version":"3.40.5"},"reference-count":51,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Risk Analysis"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Currently, there is a growing preference for convenience food products, such as ready\u2010to\u2010eat (RTE) foods, associated with long refrigerated shelf\u2010lives, not requiring a heat treatment prior to consumption. Because <jats:italic>Listeria monocytogenes<\/jats:italic> is able to grow at refrigeration temperatures, inconsistent temperatures during production, distribution, and at consumer's household may allow for the pathogen to thrive, reaching unsafe limits. <jats:italic>L. monocytogenes<\/jats:italic> is the causative agent of listeriosis, a rare but severe human illness, with high fatality rates, transmitted almost exclusively by food consumption. With the aim of assessing the quantitative microbial risk of <jats:italic>L. monocytogenes<\/jats:italic> in RTE chicken salads, a challenge test was performed. Salads were inoculated with a three\u2010strain mixture of cold\u2010adapted <jats:italic>L. monocytogenes<\/jats:italic> and stored at 4, 12, and 16 \u00b0C for eight days. Results revealed that the salad was able to support <jats:italic>L. monocytogenes\u2019<\/jats:italic> growth, even at refrigeration temperatures. The Baranyi primary model was fitted to microbiological data to estimate the pathogen's growth kinetic parameters. Temperature effect on the maximum specific growth rate (<jats:italic>\u03bc<\/jats:italic><jats:sub>max<\/jats:sub>) was modeled using a square\u2010root\u2010type model. Storage temperature significantly influenced <jats:italic>\u03bc<\/jats:italic><jats:sub>max<\/jats:sub> of <jats:italic>L. monocytogenes<\/jats:italic> (<jats:italic>p<\/jats:italic> &lt; 0.05). These predicted growth models for <jats:italic>L. monocytogenes<\/jats:italic> were subsequently used to develop a quantitative microbial risk assessment, estimating a median number of 0.00008726 listeriosis cases per year linked to the consumption of these RTE salads. Sensitivity analysis considering different time\u2013temperature scenarios indicated a very low median risk per portion (&lt;\u22127 log), even if the assessed RTE chicken salad was kept in abuse storage conditions.<\/jats:p>","DOI":"10.1111\/risa.13546","type":"journal-article","created":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T16:37:35Z","timestamp":1593621455000},"page":"2427-2441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Estimating <i>Listeria monocytogenes<\/i> Growth in Ready\u2010to\u2010Eat Chicken Salad Using a Challenge Test for Quantitative Microbial Risk Assessment"],"prefix":"10.1111","volume":"40","author":[{"given":"Rita","family":"Bernardo","sequence":"first","affiliation":[{"name":"CIISA \u2010 Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria Universidade de Lisboa, Avenida da Universidade T\u00e9cnica  Lisboa 1300\u2010477 Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1679-4461","authenticated-orcid":false,"given":"Ant\u00f3nio Salvador","family":"Barreto","sequence":"additional","affiliation":[{"name":"CIISA \u2010 Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria Universidade de Lisboa, Avenida da Universidade T\u00e9cnica  Lisboa 1300\u2010477 Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4388-5366","authenticated-orcid":false,"given":"Telmo","family":"Nunes","sequence":"additional","affiliation":[{"name":"CIISA \u2010 Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria Universidade de Lisboa, Avenida da Universidade T\u00e9cnica  Lisboa 1300\u2010477 Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9098-4389","authenticated-orcid":false,"given":"Ana Rita","family":"Henriques","sequence":"additional","affiliation":[{"name":"CIISA \u2010 Centro de Investiga\u00e7\u00e3o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterin\u00e1ria Universidade de Lisboa, Avenida da Universidade T\u00e9cnica  Lisboa 1300\u2010477 Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2015.06.004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(94)90157-0"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/fmic.1993.1005"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2016.12.016"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1541-4337.2010.00123.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-017-0956-z"},{"volume-title":"Procedural manual","year":"2015","author":"Codex Alimentarius Commission","key":"e_1_2_7_8_1"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2018.04.013"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2012.07.014"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2015.04.030"},{"issue":"22","key":"e_1_2_7_12_1","first-page":"1","article-title":"Commmission Regulation (EC) No 2073\/2005 of 15 November 2005 on microbiological criteria for foodstuffs","volume":"338","author":"Commission European.","year":"2005","journal-title":"Official Journal of the European Union"},{"issue":"12","key":"e_1_2_7_13_1","first-page":"5926","article-title":"The European Union One Health 2018 Zoonoses Report","volume":"17","author":"European Food Safety Authority (EFSA) and European Centre for Disease Prevention and Control (ECDC)","year":"2019","journal-title":"EFSA Journal"},{"issue":"1","key":"e_1_2_7_14_1","first-page":"5134","article-title":"Scientific Opinion on the Listeria monocytogenes contamination of ready\u2010to\u2010eat foods and the risk for human health in the EU","volume":"16","author":"European Food Safety Authority Panel on Biological Hazards (EFSA BioHaz)","year":"2018","journal-title":"EFSA Journal"},{"key":"e_1_2_7_15_1","unstructured":"European Union Reference Laboratory forL. monocytogenes (EURL Lm). 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