{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:41:04Z","timestamp":1790286064044,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T00:00:00Z","timestamp":1620000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Seminars in Dialysis"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Toxoplasmosis causes serious complications in immunocompromised and pregnant women. Serological tests for the detection of toxoplasmosis are often designed from parasitic tachyzoites antigens. The process of producing these antigens is very difficult. The purpose of this study was evaluation of <jats:italic>T<\/jats:italic>. <jats:italic>gondii<\/jats:italic>\u2010rGRA5 for the immunodiagnosis and molecular detection of <jats:italic>Toxoplasma<\/jats:italic> infection using enzyme\u2010linked immunosorbent assay (ELISA) and LAMP methods in hemodialysis patients. The GRA5 gene was successfully expressed and purified by affinity chromatography assay and evaluated by western blot. Then it was used to design an ELISA assay. A total of 260 samples were tested for anti\u2010<jats:italic>Toxoplasma<\/jats:italic> IgG and IgM antibodies using a commercial ELISA kit and designed ELISA kit. Finally, the <jats:italic>LAMP<\/jats:italic> method was used to evaluate the precision and reliability of the results obtained by commercial and designed ELISA kits. The consistency of the results of two methods was analyzed using the Kappa coefficient of agreement. The rGRA5 revealed higher immunoreactivity with 1:100 dilution of sera from toxoplasmosis patients. The specificity and sensitivity of the assay were 93% and 96%, respectively. According to the Kappa coefficient, there was a substantial correlation between the results of ELISA and LAMP based on rGRA5 (\u224898%, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). Also it showed that rGRA5 protein can be used as an antigenic protein for designing sero\u2010diagnostic tests to identify <jats:italic>Toxoplasma<\/jats:italic> infection especially in hemodialysis patients.<\/jats:p>","DOI":"10.1111\/sdi.12981","type":"journal-article","created":{"date-parts":[[2021,5,3]],"date-time":"2021-05-03T20:37:26Z","timestamp":1620074246000},"page":"332-337","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Immunodiagnosis and molecular validation of <i>Toxoplasma gondii<\/i>\u2010recombinant dense granular (GRA) 5 protein for the detection of toxoplasmosis in hemodialysis patients"],"prefix":"10.1111","volume":"34","author":[{"given":"Zahra","family":"Arab\u2010Mazar","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Public Health Tehran University of Medical Sciences  Tehran Iran"},{"name":"Infectious Diseases and Tropical Medicine Research Center Shahid Beheshti University of Medial Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amirreza","family":"Javadi Mamaghani","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shirzad","family":"Fallahi","sequence":"additional","affiliation":[{"name":"Razi Herbal Medicines Research Center Lorestan University of Medical Science  Khorramabad Iran"},{"name":"Department of Medical Parasitology and Mycology Faculty of Medicine Lorestan University of Medical Sciences  Khorramabad, Iran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simin","family":"Rajaeian","sequence":"additional","affiliation":[{"name":"Department of Applied Cell Sciences School of advanced Technologies in Medicine Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameneh","family":"Koochaki","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Faculty of Medicine Shahid Beheshti University of Medical Sciences  Tehran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9061-5089","authenticated-orcid":false,"given":"Seyyed Javad","family":"Seyyed Tabaei","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology School of Medicine Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanieh","family":"Rezaee","sequence":"additional","affiliation":[{"name":"Department of Biotechnology School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences  Tehran 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