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Groups (<jats:italic>n\u00a0<\/jats:italic>=\u00a06\u201312) of inbred and outbred mouse strains (Balb\/c, C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6, <jats:styled-content style=\"fixed-case\">OFI<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NMRI<\/jats:styled-content>) were immunized with the two vaccines, and the induced antibody levels and bactericidal and opsonic activities measured. Except for Balb\/c mice, which were low responders, the genetically modified vaccine raised high Omp85 antibody levels in all mouse strains. In comparison, the wild\u2010type vaccine gave lower antibody levels, but <jats:styled-content style=\"fixed-case\">NMRI<\/jats:styled-content> mice responded to this vaccine with the same high levels as the modified vaccine in the other strains. Although the vaccines induced strain\u2010dependent Omp85 antibody responses, the mouse strains showed high and similar serum bactericidal titres. Titres were negligible with heterologous or PorA\u2010negative meningococcal target strains, demonstrating the presence of the dominant bactericidal PorA antibodies. The two vaccines induced the same opsonic titres. Thus, the genetically modified vaccine with high Omp85 antibody levels and the wild\u2010type vaccine induced the same levels of functional activities related to protection against meningococcal disease, suggesting that meningococcal Omp85 is a less attractive vaccine antigen.<\/jats:p>","DOI":"10.1111\/sji.12051","type":"journal-article","created":{"date-parts":[[2013,3,23]],"date-time":"2013-03-23T22:04:17Z","timestamp":1364076257000},"page":"452-459","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Meningococcal Omp85 in Detergent\u2010Extracted Outer Membrane Vesicle Vaccines Induces High Levels of Non\u2010Functional Antibodies in Mice"],"prefix":"10.1111","volume":"77","author":[{"given":"E.","family":"Wedege","sequence":"first","affiliation":[{"name":"Division of Infectious Disease Control Department of Bacteriology and Immunology Norwegian Institute of Public Health Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Lie","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease Control Department of Bacteriology and Immunology Norwegian Institute of Public Health Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Bolstad","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease Control Department of Bacteriology and Immunology Norwegian Institute of Public Health Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. 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