{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T12:04:23Z","timestamp":1775304263404,"version":"3.50.1"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,7,11]],"date-time":"2013-07-11T00:00:00Z","timestamp":1373500800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2013,7]]},"abstract":"Abstract<\/jats:title>Brucellosis remains a major zoonosis worldwide. Brucella antigens induce the production of T\u2010helper 1 (Th1) cytokines such as interleukin\u201012 (IL<\/jats:styled-content>\u201012) in humans. We aimed to investigate the association of two single nucleotide polymorphisms (SNP<\/jats:styled-content>s) in the gene encoding the IL<\/jats:styled-content>\u201012p40 cytokine (IL<\/jats:styled-content>\u201012B) with brucellosis and to examine the functionality of these SNP<\/jats:styled-content>s through measuring serum levels of IL<\/jats:styled-content>\u201012p40. We genotyped IL<\/jats:styled-content>\u201012B<\/jats:italic> gene rs3212227, A>C; rs6887695\u00a0G>C polymorphisms in a case\u2010control study on a total of 281 subjects including 153 patients with active brucellosis and 128 healthy controls, using RFLP<\/jats:styled-content> and serum IL<\/jats:styled-content>\u201012p40 levels, were assessed by ELISA<\/jats:styled-content>. The rs3212227 minor allele (C) and homozygote genotype (CC<\/jats:styled-content>) were more frequent in controls compared with patients with brucellosis (P<\/jats:italic>\u00a0=\u00a00.006, OR<\/jats:styled-content>\u00a0=\u00a00.608, 95%CI<\/jats:styled-content>\u00a0=\u00a00.429\u20130.861 for the C allele; P<\/jats:italic>\u00a0=\u00a00.024, OR<\/jats:styled-content>\u00a0=\u00a00.443, 95% CI<\/jats:styled-content>: 0.218\u20130.900 for the CC<\/jats:styled-content> genotype). Comparison of IL<\/jats:styled-content>\u201012B<\/jats:italic> genotypes and serum levels of the IL<\/jats:styled-content>\u201012p40 revealed that rs3212227 AA<\/jats:styled-content> genotype, with higher frequency in patients than in controls, was associated with increased levels of the cytokine (P<\/jats:italic>\u00a0=\u00a00.0001). Furthermore, the distribution of haplotype and genotype combinations in our study suggested that rs3212227C\/rs6887695C haplotype or CC<\/jats:styled-content>\/GC<\/jats:styled-content> or CC<\/jats:styled-content>\/CC<\/jats:styled-content> genotype combinations may protect controls against Brucella infection by contributing to a functional downregulation of the serum IL<\/jats:styled-content>\u201012p40 production in vivo<\/jats:italic>, as shown by ELISA<\/jats:styled-content> (P<\/jats:italic>\u00a0<\u00a00.05). Overall, our study demonstrated that rs3212227 A variant was associated with higher levels of serum IL<\/jats:styled-content>\u201012p40 and could possibly contribute to an inherited predisposition to brucellosis.<\/jats:p>","DOI":"10.1111\/sji.12054","type":"journal-article","created":{"date-parts":[[2013,4,12]],"date-time":"2013-04-12T05:12:42Z","timestamp":1365743562000},"page":"85-91","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Levels of Interleukin\u2010(IL<\/scp>)\u201012p40 are Markedly Increased in Brucellosis Among Patients with Specific IL<\/scp>\u201012B<\/i> Genotypes"],"prefix":"10.1111","volume":"78","author":[{"given":"E.","family":"Eskandari\u2010Nasab","sequence":"first","affiliation":[{"name":"Infectious Diseases and Tropical Medicine Research Center Zahedan University of Medical Sciences Zahedan Iran"},{"name":"Department of Clinical Biochemistry School of Medicine Zahedan University of 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