{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T20:47:03Z","timestamp":1770670023287,"version":"3.49.0"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T00:00:00Z","timestamp":1378944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The function of cultured mast cells may depend on genetic or environmental influence on the stem cell donor. This study investigates whether asthma or atopy in the donor influenced the growth and sensitivity of mast cells cultured from patients with asthma and healthy controls under identical conditions. Mast cells were cultured from peripheral blood from twelve patients with an objectively confirmed asthma diagnosis and eight healthy subjects. During the last 2\u00a0weeks of culture, mast cells were incubated with <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104 and 80\u00a0kU\/l recombinant human <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> containing two clones (7%\u00a0+\u00a07%) specific for mite allergen <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>er p2. The sensitivity of <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>\u2010mediated activation of mast cells was investigated as <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>c\u03b5<jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>\u2010mediated upregulation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63. Ten subjects were atopic, defined as a positive skin prick test (&gt;3\u00a0mm) to at least one of ten common allergens. After activation with recombinant <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>er p2, the maximum <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 median fluorescence intensity was 20 456\u00a0\u00b1\u00a01640 (<jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content>) for patients with asthma and 22 275\u00a0\u00b1\u00a01971 (<jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content>) for controls (ns). The fraction of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 positive cells was 54.4% in patients with asthma and 48.4% in controls (ns). The allergen concentration inducing 50% of the maximal <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 response was similar in patients with asthma [\u22120.4795 log ng\/ml\u00a0\u00b1\u00a00.092 (<jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content>)] and controls (\u22120.6351 log ng\/ml\u00a0\u00b1\u00a00.083, ns) and in atopic and non\u2010atopic subjects. When cultured, sensitized and activated under identical conditions, mast cells from allergic asthmatics and healthy controls respond similar. Activation of cultured mast cells appears to depend on culture conditions (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104, <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>) rather than on donor status as atopy and asthma.<\/jats:p>","DOI":"10.1111\/sji.12085","type":"journal-article","created":{"date-parts":[[2013,6,24]],"date-time":"2013-06-24T05:16:38Z","timestamp":1372050998000},"page":"352-356","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Cultured Mast Cells from Patients with Asthma and Controls Respond with Similar Sensitivity to Recombinant <scp>D<\/scp>er <scp>P<\/scp>2\u2010Induced, <scp>I<\/scp>g<scp>E<\/scp>\u2010Mediated Activation"],"prefix":"10.1111","volume":"78","author":[{"given":"I. K.","family":"Krohn","sequence":"first","affiliation":[{"name":"Department of Pulmonary Medicine Aarhus University Hospital Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Sverrild","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine L Bipsebjerg University Hospital Bispebjerg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Lund","sequence":"additional","affiliation":[{"name":"ALK\u2010Abello H\u00f8rsholm Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Dahl","sequence":"additional","affiliation":[{"name":"Institute for Clinical Medicine, Health Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. S.","family":"Erjef\u00e4lt","sequence":"additional","affiliation":[{"name":"Airway Inflammation Unit Department of Experimental Medical Sciences Lund University Lund Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Backer","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine L Bipsebjerg University Hospital Bispebjerg Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. J.","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine Aarhus University Hospital Aarhus Denmark"},{"name":"Institute for Clinical Medicine, Health Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"3705","DOI":"10.1182\/blood.V86.10.3705.bloodjournal86103705","article-title":"Effects of T\u2010helper 2\u2010type cytokines, interleukin\u20103 (IL\u20103), IL\u20104, IL\u20105, and IL\u20106 on the survival of cultured human mast cells","volume":"86","author":"Yanagida M","year":"1995","journal-title":"Blood"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2004.01.006"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/8.9.1367"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2007.02.003"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000328756"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri914"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.05.026"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(91)90094-5"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/02770903.2012.752504"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2008.04.016"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2008.04.019"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00138707"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.07.053"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2008.101683"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113234"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.01.022"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02723.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb08450.x"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425043"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(90)90153-U"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02051.x"}],"container-title":["Scandinavian Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fsji.12085","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/sji.12085","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T15:01:55Z","timestamp":1696172515000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/sji.12085"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,12]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1111\/sji.12085"],"URL":"https:\/\/doi.org\/10.1111\/sji.12085","archive":["Portico"],"relation":{},"ISSN":["0300-9475","1365-3083"],"issn-type":[{"value":"0300-9475","type":"print"},{"value":"1365-3083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,12]]},"assertion":[{"value":"2013-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}