{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T02:23:41Z","timestamp":1783995821802,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,11,28]],"date-time":"2013-11-28T00:00:00Z","timestamp":1385596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tolerogenic dendritic cells (<jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s) potently induce and maintain tolerance based on their distinct characteristics compared with conventional DCs. Recent reports show that donor or host <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s promote allograft survival in mice. In this study, the efficacy of third\u2010party <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s in the prevention of acute graft\u2010versus\u2010host disease (<jats:styled-content style=\"fixed-case\">aGVHD<\/jats:styled-content>) was evaluated. <jats:italic>In vitro<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> tDC<\/jats:styled-content>s derived from the bone marrow (BM) of D1 mice were induced by GM\u2010CSF, IL\u201010 and TGF\u2010\u03b21. The phenotypes, expression of cytokines and suppression of <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s were analysed. <jats:italic>In vivo<\/jats:italic>, the effects of adoptive transfer of third\u2010party\u2010<jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s were evaluated in an MHC\u2010mismatched <jats:styled-content style=\"fixed-case\">aGVHD<\/jats:styled-content> mouse model. Survival, body weight, GVHD scoring, histopathological specimens and serum cytokines were analysed in <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>\u2010treated mice and untreated controls. Tolerogenic DCs had low expression of MHC and co\u2010stimulatory molecules, expressed high levels of \u2018immunosuppressive\u2019 cytokines and suppressed allo\u2010CD4<jats:sup>+<\/jats:sup>T cell proliferation. In the B6\u2192D2 mouse model, all <jats:styled-content style=\"fixed-case\">aGVHD<\/jats:styled-content> mice died within 18\u00a0days. Fortunately, third\u2010party <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s transferred at low doses (10<jats:sup>4<\/jats:sup>) effectively prolonged survival after allo\u2010BMT. Furthermore, in the mice treated with 10<jats:sup>4<\/jats:sup> <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s, serum levels of IL\u201010\/TGF\u2010\u03b2 were significantly higher and the percentage of Foxp3<jats:sup>+<\/jats:sup> cells continually increased compared with the mice treated with other doses of <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s. Third\u2010party <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s play a crucial role in reducing the severity of <jats:styled-content style=\"fixed-case\">aGVHD<\/jats:styled-content> by modulating the secretion of various cytokines and expanding Foxp3<jats:sup>+<\/jats:sup> regulatory T cells, which suggests the possibility of using third\u2010party <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s for therapeutic applications. Furthermore, special attention should be paid to the optimal range of <jats:styled-content style=\"fixed-case\">tDC<\/jats:styled-content>s for preventing allograft rejection.<\/jats:p>","DOI":"10.1111\/sji.12113","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T21:56:13Z","timestamp":1381528573000},"page":"486-496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Third\u2010party Tolerogenic Dendritic Cells Reduce Allo\u2010reactivity <i>In vitro<\/i> and Ameliorate the Severity of Acute graft\u2010Versus\u2010host Disease in Allo\u2010bone Marrow Transplantation"],"prefix":"10.1111","volume":"78","author":[{"given":"J.","family":"Yang","sequence":"first","affiliation":[{"name":"Blood Engineering Laboratory Shanghai Blood Center Shanghai China"},{"name":"Division of Rheumatology Huashan Hospital Fudan University Shanghai 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