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Therefore, more efficacious vaccines are urgently needed. In this study, we constructed a new tuberculosis vaccine of recombinant BCG strain (<jats:styled-content style=\"fixed-case\">rBCG<\/jats:styled-content>\u2010IA), which could express IL\u201012p70 of human cytokine and Ag85A of <jats:italic>M.\u00a0tuberculosis<\/jats:italic> fusion protein, and investigated its immunogenicity in BALB\/c mice by measuring antibody titres, proliferation rate of splenocytes, ratios of CD4<jats:sup>+<\/jats:sup> T and CD8<jats:sup>+<\/jats:sup> T cells stimulated by specific antigens and levels of IFN\u2010\u03b3 production in antigen\u2010stimulated splenocyte cultures. Meanwhile, we evaluated its protective efficacy against <jats:italic>M.\u00a0tuberculosis<\/jats:italic> H37Rv infection through detecting lung histopathology, organ bacterial loads and lung acid\u2010fast stain. Immunogenicity experiments illustrated that from 2nd to 8th week after immunization, the <jats:styled-content style=\"fixed-case\">rBCG<\/jats:styled-content>\u2010IA vaccine was able to induce the highest level of antibody titres, proliferation rate of splenocytes and IFN\u2010\u03b3 production among groups and gained improved ratio of CD4<jats:sup>+<\/jats:sup> T and CD8<jats:sup>+<\/jats:sup> T cells from 6th to 8th week after vaccination. And from 2nd to 8th week after <jats:italic>M.\u00a0tuberculosis<\/jats:italic> H37Rv infection, the score of pathology and bacterial loads in the <jats:styled-content style=\"fixed-case\">rBCG<\/jats:styled-content>\u2010IA group were obviously lower than that in <jats:styled-content style=\"fixed-case\">rBCG<\/jats:styled-content>\u2010I group, <jats:styled-content style=\"fixed-case\">rBCG<\/jats:styled-content>\u2010A group or control group (PBST group), but similar to that in BCG group. This study suggested that <jats:styled-content style=\"fixed-case\">rBCG<\/jats:styled-content>\u2010IA was able to elicit stronger humoral and cellular immune responses, but could only confer similar protective efficacy compared with its parental BCG vaccine.<\/jats:p>","DOI":"10.1111\/sji.12116","type":"journal-article","created":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T21:51:32Z","timestamp":1381528292000},"page":"497-506","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Immunogenicity and Protective Efficacy Conferred by a Novel Recombinant <i>Mycobacterium bovis<\/i> Bacillus Calmette\u2010Gu\u00e9rin Strain Expressing Interleukin\u201012p70 of Human Cytokine and Ag85A of <i>Mycobacterium tuberculosis<\/i> Fusion Protein"],"prefix":"10.1111","volume":"78","author":[{"given":"Y. 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