{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T14:31:33Z","timestamp":1784817093146,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,12,19]],"date-time":"2013-12-19T00:00:00Z","timestamp":1387411200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>During the last decade, cases of the fish parasite <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>nisakis simplex<\/jats:italic> infection and allergy in human have increased in countries with high fish consumption. Our aim was to perform an extended seroprevalence study of anti\u2010IgE antibodies against this parasite in <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>orway, one of the high fish\u2010consuming countries. At the Department of Immunology and Transfusion Medicine and the Laboratory of Clinical Biochemistry, Haukeland University Hospital, <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>ergen, <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>orway, two main groups of anonymized serum samples were collected; the first (<jats:italic>n<\/jats:italic>\u00a0=\u00a0993) from recently recruited blood donors (designated \u2018<jats:styled-content style=\"fixed-case\">BDO<\/jats:styled-content>\u2019) and the second (<jats:italic>n<\/jats:italic>\u00a0=\u00a0414) from patient with total IgE levels \u22651000\u00a0kU\/l (designated \u2018IGE+\u2019). The sera were analysed by the ImmunoCAP<jats:sup>\u00ae<\/jats:sup> method for total IgE and IgE antibodies against <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0simplex<\/jats:italic>, house dust mite (<jats:styled-content style=\"fixed-case\">HDM<\/jats:styled-content>), shrimp, cod, crab, brine shrimp and shrimp tropomyosin. The <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0simplex<\/jats:italic> positive sera were further tested by an enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) method, which uses 2 recombinant (r) major allergens, rAni s 1 and rAni s 7 as target antigens. <jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content> and Western immunoblotting analyses were also performed. Whereas the prevalences by ImmunoCAP<jats:sup>\u00ae<\/jats:sup> were 0.4% and 16.2% in the <jats:styled-content style=\"fixed-case\">BDO<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">IGE<\/jats:styled-content>+ groups, respectively, analyses with recombinant allergens showed only 0.0% and 0.2%. Cross\u2010reactivity and immunoblotting analyses suggested that most of the ImmunoCAP<jats:sup>\u00ae<\/jats:sup> positive sera were probably false\u2010positive due to cross\u2010sensitization to shrimp and <jats:styled-content style=\"fixed-case\">HDM<\/jats:styled-content>. However, positivity due to other <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>.\u00a0simplex<\/jats:italic> antigens should also be considered. Compared with other high fish\u2010consuming countries, we observed a very low seroprevalence of anti\u2010<jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>nisakis<\/jats:italic> IgE antibodies in a <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>orwegian population.<\/jats:p>","DOI":"10.1111\/sji.12130","type":"journal-article","created":{"date-parts":[[2013,11,13]],"date-time":"2013-11-13T11:29:00Z","timestamp":1384342140000},"page":"61-67","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["An Extended Study of Seroprevalence of Anti\u2010<i><scp>A<\/scp>nisakis simplex<\/i> IgE Antibodies in <scp>N<\/scp>orwegian Blood Donors"],"prefix":"10.1111","volume":"79","author":[{"given":"A. 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