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However, a booster vaccination of subunit vaccine on week 3 after primary immunization elicited a significantly higher antibody titre than a single dose, whereas no significant antibody titre difference was observed between a single dose and two doses of <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>76 vaccination. To address these issues, in this study, we first investigated the kinetics of memory <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells and plasma cells in the mice immunized with <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>76 or F1 protein by flow cytometry and then determined antibody titre in five groups of mice immunized with various vaccination strategy. The results showed that memory <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells dropped to a low level at day 56 after primary immunization. In contrast, plasma cells were maintained for more than 98\u00a0days. The group with primary immunization of <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>76 and booster of F1 antigen developed a higher antibody titre than the group with immunization of F1 antigen and booster of <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>76. This result supports a hypothesis that an excess of antigens can neutralize pre\u2010existing antibodies, and then the redundant antigen induces antibody boost. Taken together, a boost of antibody titre after revaccination may be dependent on the existence of memory <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells and an excess of antigen vaccination. In addition, this study showed an ideal immunization strategy that involves first immunization with a live attenuated vaccine, such as <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>76, and then with a subunit vaccine.<\/jats:p>","DOI":"10.1111\/sji.12146","type":"journal-article","created":{"date-parts":[[2014,1,2]],"date-time":"2014-01-02T18:34:43Z","timestamp":1388687683000},"page":"157-162","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Kinetics of Memory <scp>B<\/scp> Cell and Plasma Cell Responses in the Mice Immunized with Plague Vaccines"],"prefix":"10.1111","volume":"79","author":[{"given":"X.","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory of Analytical Microbiology State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 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